 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Nov 01, 2022 |
| Title |
RNA sequencing of intestinal enterocytes pre- and post-Roux-en-Y gastric bypass reveals alteration in gene expression related to enterocyte differentiation, restitution, and obesity |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Background: The intestinal lining renews itself in a programmed fashion that can be affected by adaptation to surgical procedures such as gastric bypass.
Results: RNA sequencing suggested significant decreases of gene expression associated with G2/M DNA damage checkpoint regulation of the cell cycle pathway, and significant increases in gene expression associated with CDP-diacylglycerol biosynthesis pathway TCA cycle II pathway, and pyrimidine ribonucleotide salvage pathway after RYGB. Since SLFN12 is reported to influence enterocytic differentiation, we stained mucosa for SLFN12 and observed increased SLFN12 immunoreactivity. We investigated SLFN12 overexpression in HIEC-6 and FHs 74 Int intestinal epithelial cells and observed similar increased expression of the following genes that were also increased after RYGB: HES2, CARD9, SLC19A2, FBXW7, STXBP4, SPARCL1, and UTS.
Conclusions: Our data suggests that RYGB promotes SLFN12 protein expression, cellular mechanism and replication pathways, and genes associated with differentiation and restitution (HES2, CARD9, SLC19A2) and obesity-related genes (FBXW7, STXBP4, SPARCL1, UTS).
|
| |
|
| Overall design |
Methods: To assess adaptive mechanisms in the human intestine after Roux-en-Y gastric bypass (RYGB), we biopsied proximal jejunum at the anastomotic site at surgery to establish a baseline and endoscopically re-biopsied the same area 6-9 months after bypass for comparison. Laser microdissection was performed on pre- and post-RYGB biopsies to isolate enterocytes for RNA sequencing.
|
| |
|
| Contributor(s) |
Vomhof-DeKrey EE, Singhal S, Singhal S, Stover A, Rajpathy O, Preszler E, Garcia L, Basson M |
| Citation(s) |
36291149 |
| |
| Submission date |
Oct 04, 2022 |
| Last update date |
Dec 15, 2022 |
| Contact name |
Sandeep K Singhal |
| E-mail(s) |
Sandeep.singhal@und.edu
|
| Organization name |
University of North Dakota
|
| Department |
Pathology
|
| Street address |
1301 N Columbia Rd Stop 9037
|
| City |
Grand Forks |
| State/province |
NORTH DAKOTA |
| ZIP/Postal code |
58202 |
| Country |
USA |
| |
|
| Platforms (1) |
|
| Samples (22)
|
|
| Relations |
| BioProject |
PRJNA887029 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE214758_Raw_counts.txt.gz |
9.3 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
|
|
|
|
 |
Less...
More...