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| Status |
Public on Apr 04, 2024 |
| Title |
The Myc-Associated Zinc Finger Protein (MAZ) controls STAT1-mediated antiviral response by reshaping epigenome [MeDIP-Seq] |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by high throughput sequencing
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| Summary |
The objectives of this study are to understand the regulatory roles of MAZ in biological processes using the NGS-deriveed ChIP-seq, DNA-MEDIP-seq and RNA-seq data in HAP1 control cells and MAZ knockout cells. Our comparative analysis of these data generated from the HAP1 control and MAZ KO cells shows that MAZ is required for recruiting STAT1 to its target sites by reshaping epigenetic landscape in the human genome, thereby mediating antiviral response cells.
MEDIP-seq was performed using the MagMeDIP-seq Package from Diagenode in HAP1 control and HAP1 MAZ knockout cells following manufacturer's instructions.
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| Overall design |
MeDIP-seq data of HAP1 control cells and MAZ kockout cells were generated by deep sequencing in duplicate using Illumina GAIIx. The MEDIP-seq sequence reads were aligned with Bowtie2 followed by calling peaks with MACS2 software. Distribution of DNA methylation was analyzed using Deeptools and other software.
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| Contributor(s) |
Xiao T, Li X, Felsenfeld G |
| Citation(s) |
38635637 |
| |
| Submission date |
Oct 08, 2022 |
| Last update date |
Jul 02, 2024 |
| Contact name |
Gary Felsenfeld |
| E-mail(s) |
txiao2015@gmail.com
|
| Organization name |
NIH
|
| Lab |
LMB
|
| Street address |
9000 Rockville Pike
|
| City |
Bethesda |
| ZIP/Postal code |
20892 |
| Country |
USA |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (4)
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| GSM6621011 |
DNA-meIP-HAP1-p-lib1-922-2021_S1_L004 |
| GSM6621012 |
DNA-meIP-HAP1-p-IFN-lib2-922-2021_S4_L004 |
| GSM6621013 |
DNA-meIP-HAP1-M-lib3-922-2021_S7_L004 |
| GSM6621014 |
DNA-meIP-HAP1-M-IFN-lib4-922-2021_S2_L004 |
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| This SubSeries is part of SuperSeries: |
| GSE215083 |
The Myc-Associated Zinc Finger Protein (MAZ) controls STAT1-mediated antiviral response by reshaping epigenome |
|
| Relations |
| BioProject |
PRJNA888375 |
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