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| Status |
Public on Nov 29, 2022 |
| Title |
Live or heat-killed Lactobacillus rhamnosus aerosolization decreases adenomatous lung cancer development in a mouse carcinogen-induced tumor model |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
One of the responsible factors involved in the progression of pre-malignant lung lesions to lung cancer is the increased immunosuppressive microenvironment of the lung, that is deeply infleunced by the microbiota. In this article, we have investigated if the perturbation of lung microbiota by antibiotics or probiotic aerosol, interferes with lung cancer development in a mouse carcinogen-induced tumor model. Specifically, the effect is mostly observed with aerosol with Lactobacillus Rhamnosus GG that results to impair lung cancer growth by promoting local immunity. This study points out to new and non-invasive strategy to treat individuals at risk of lung cancer.
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| Overall design |
Urethane-injected mice were vancomycin/neomycin (V/N)- or live- or dead- L. Rhamnosus GG (L.RGG)- aerosolized and tumor development was evaluated. Transcriptional profiling of lungs and IHC were performed. Mice were sacrificed, tumor nodules were collected, and RNA was extracted using the RNeasy Mini Kit (Qiagen) for the gene expression profile. In L.RGG-treated groups the gene encoding the joining chain (JChain) of immunoglobulins was significantly increased.
LRGG M = heat-killed LRGG
LRGG V = live LRGG
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| Contributor(s) |
De Cecco L |
| Citation(s) |
36361537 |
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| Submission date |
Oct 12, 2022 |
| Last update date |
Nov 30, 2022 |
| Contact name |
Loris De Cecco |
| E-mail(s) |
loris.dececco@istitutotumori.mi.it
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| Organization name |
IRCSS Istituto Nazionale Tumori
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| Street address |
via Venezian 1
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| City |
Milan |
| ZIP/Postal code |
20133 |
| Country |
Italy |
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| Platforms (1) |
| GPL23038 |
[Clariom_S_Mouse] Affymetrix Clariom S Assay, Mouse (Includes Pico Assay) |
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| Samples (36)
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| Relations |
| BioProject |
PRJNA889756 |
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