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| Status |
Public on Oct 18, 2022 |
| Title |
A divergent and complementary transcriptional response in poxvirus-infected and bystander inflammatory monocytes is partly dictated by interferon [iMO IFN ECTV] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Inflammatory monocytes (iMO) and B-cells are the main targets of the poxvirus ectromelia virus (ECTV) in the lymph nodes of mice and play distinct roles in surviving the infection. Infected and bystander iMO control ECTV’s systemic spread, preventing early death, while B-cells make antibodies that eliminate ECTV. Our work demonstrates that within an infected animal that survives ECTV infection, intrinsic and bystander infection of iMO and B-cells differentially control the transcription of genes important for immune cell function and, perhaps, cell identity. Bystander cells upregulate metabolism, antigen presentation, and interferon-stimulated genes. Infected cells downregulate many cell-type-specific genes and upregulate transcripts typical of non-immune cells. Bys and Inf iMO non-redundantly contribute to the cytokine milieu and the interferon response. Furthermore, we uncovered how IFN-I or IFN-γ signaling differentially regulates immune pathways in Inf and Bys iMO and, that at steady state, IFN-I primes iMO for rapid IFN-I production and antigen presentation.
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| Overall design |
Bystander and infected iMO were sorted from popliteal draining lymph nodes of ectromelia virus-infected mixed bone marrow chimeric mice at 3 days post-infection. GFP-expressing ectromelia virus was used for sorting Bystander and Infected cells. As controls, Naïve iMO were sorted from spleens of uninfected bone marrow chimeras. 4 biological replicates were sequenced for WT bystander and infected iMO. 2 biological replicates were sequenced for Naive iMO and for knockout bystander and infected iMO.
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| Web link |
https://pubmed.ncbi.nlm.nih.gov/36417857/
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| Contributor(s) |
Melo-Silva CR, Roman MI, Knudson CJ, Tang L, Xu R, Tassetto M, Dolan P, Andino R, Sigal LJ |
| Citation(s) |
36417857 |
| |
| Submission date |
Oct 14, 2022 |
| Last update date |
Jan 17, 2023 |
| Contact name |
Carolina Rezende Melo da Silva |
| E-mail(s) |
Carolina.Rezende.Melo.da.Silva@jefferson.edu
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| Phone |
2675742336
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| Organization name |
Thomas Jefferson University
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| Department |
Microbiology and Immunology
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| Street address |
2527 Morgan run
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| City |
Huntingdon Valley |
| State/province |
Pennsylvania |
| ZIP/Postal code |
19006 |
| Country |
USA |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (22)
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| This SubSeries is part of SuperSeries: |
| GSE215747 |
A divergent and complementary transcriptional response in poxvirus-infected and bystander inflammatory monocytes is partly dictated by interferon. |
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| Relations |
| BioProject |
PRJNA890549 |
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