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| Status |
Public on Dec 12, 2023 |
| Title |
ONT-cappable-seq for P23-45 bacteriophage 5 min post-infection |
| Organism |
Thermus thermophilus HB8 |
| Experiment type |
Expression profiling by high throughput sequencing
Other
|
| Summary |
We performed the long-read RNA sequencing technique ONT-cappable-seq on RNA samples of T. thermophilus infected with phage P23-45 5 minutes post-infection. Using this approach, we obtained the primary transcriptome at the early infection stage and sequenced it in full-length. Based on this data, we were able to identify viral transcription start sites and termination sites and uncover distinct promoter motifs.
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| Overall design |
Full-length transcriptional profiling of T. thermophilus infected with phage P23-45 at 5min post-infection using ONT-cappable-seq.
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| Contributor(s) |
Putzeys L, Boon M, Lavigne R, Sokolova M |
| Citation(s) |
38182597 |
| |
| Submission date |
Oct 14, 2022 |
| Last update date |
Jan 17, 2024 |
| Contact name |
Rob Lavigne |
| E-mail(s) |
rob.lavigne@kuleuven.be
|
| Organization name |
KU Leuven
|
| Department |
Biosystems
|
| Lab |
Laboratory of Gene Technology
|
| Street address |
Kasteelpark Arenberg 21
|
| City |
Leuven |
| ZIP/Postal code |
3001 |
| Country |
Belgium |
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| Platforms (1) |
| GPL32746 |
PromethION (Thermus thermophilus HB8) |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE215812 |
T. thermophilus infected with phage P23-45 |
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| Relations |
| BioProject |
PRJNA890649 |
