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Series GSE215973 Query DataSets for GSE215973
Status Public on Oct 21, 2022
Title Differential gene expression analysis of PTGS2-inducing and PTGS2-non-inducing oropharyngeal carcinoma cell lines.
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Prostaglandin E2 (PGE2) is a powerful immunomodulator synthesized by the COX2 enzyme, coded by human gene PTGS2. PGE2 is known to possess angiogenic, protumor and immunosuppressive functionality in the microenviornment of oropharyngeal carcinomas (OPC). Differential induction of PTGS2 in infiltrating myeloid-derived immunocytes has been observed with media conditioned by cultured OPC biopsies and OPC-derived cell lines alike. To identify PTGS2-inducing, OPC-derived gene products, we utilized bluk RNA sequencing on 4 OPC-derived cell lines to compare expression profiles of PTGS2-inducing (SCC154, SCC25) with that of PTGS2-non-inducing (2A3, SCC152) cell lines. Interleukin-1α (IL-1α) was among the differentially expressed candidates between cell lines.
 
Overall design Comparative gene expression profiling analysis of RNA-seq data of 2A3 and SCC152 vs. SCC154 and SCC25 cell lines.
 
Contributor(s) Bonagura VR, Steinberg BM, DeVoti JA, Israr M, Lam F, Papayannakos CJ
Citation(s) 36426368
Submission date Oct 18, 2022
Last update date Dec 09, 2022
Contact name Vincent R Bonagura
E-mail(s) vbonagura@northwell.edu
Organization name Northwell Health
Department Pediatric Immunology
Lab Bonagura
Street address 350 Community Drive
City Manhasset
State/province New York
ZIP/Postal code 11030
Country USA
 
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (4)
GSM6652285 ATCC cell line CRL-3212 2A3
GSM6652286 ATCC cell line CRL-3240 UPCI:SCC152
GSM6652287 ATCC cell line CRL-3241 UPCI:SCC154
Relations
BioProject PRJNA891613

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Supplementary file Size Download File type/resource
GSE215973_Partek_OPC_cells_lines_CP2021_DESeq2_Yes_vs_No.txt.gz 1.7 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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