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| Status |
Public on Feb 26, 2024 |
| Title |
Single-cell chromatin landscape of non-myocytes reveals AP-1 as a key transcriptional regulator contributing to highly cooperative networks among non-myocyte subtypes during zebrafish heart regeneration [scATAC-seq] |
| Organism |
Danio rerio |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We applied single-cell Assay for Transposase Accessible Chromatin using sequencing (scATAC-seq) to determine the intrinsic genetic programs underlying nonCM subpopulation determination during zebrafish heart regeneration at a single cell level. A total of approximately 13,000 single non-CMs derived from zebrafish hearts isolated at different time points during heart regeneration were measured and defined into diverse cell populations, exploited for cell type-specific cis-regulatory elements and enriched motifs as control catalog of downstream cell-state-determining transcription factors.
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| Overall design |
To assess the dynamics of chromatin lanscape of non-CMs during zebrafish heart regenration, we collected the hearts from 0, 2, 7, 14 days post amputation and lysis the nuclei to perform scATAC-seq.
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| Contributor(s) |
Dong Y, Yang Y, Liu J |
| Citation(s) |
38381829 |
| |
| Submission date |
Oct 18, 2022 |
| Last update date |
Feb 26, 2024 |
| Contact name |
LI QIAN |
| E-mail(s) |
li_qian@med.unc.edu
|
| Phone |
919 9624982
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| Organization name |
UNC
|
| Department |
Department of pathology
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| Lab |
Qian Lab
|
| Street address |
111 Mason Farm Road, MBRB
|
| City |
Chapel Hill |
| ZIP/Postal code |
27517 |
| Country |
USA |
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| Platforms (1) |
| GPL20828 |
Illumina NextSeq 500 (Danio rerio) |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE215988 |
Single-cell chromatin landscape of non-myocytes reveals AP-1 as a key transcriptional regulator contributing to highly cooperative networks among non-myocyte subtypes during zebrafish heart regeneration |
|
| Relations |
| BioProject |
PRJNA891665 |
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