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| Status |
Public on Oct 19, 2022 |
| Title |
Memory phase-specific genes identified by CrebB-target DamID in the Mushroom Bodies |
| Organism |
Drosophila melanogaster |
| Experiment type |
Other
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| Summary |
The formation of long-term memories requires changes in the transcriptional program and de novo protein synthesis. One of the critical regulators for long-term memory (LTM) formation and maintenance is the transcription factor CREB. Genetic studies have dissected the requirement of CREB activity within memory circuits, however less is known about the genetic mechanisms acting downstream of CREB and how they may contribute defining LTM phases. To better understand the downstream mechanisms, we here used a targeted DamID approach (TaDa). We generated a CREB-Dam fusion protein using the fruit fly Drosophila melanogaster as model. Expressing CREB-Dam in the mushroom bodies (MBs), a brain center implicated in olfactory memory formation, we identified genes that are differentially expressed between paired and unpaired appetitive training paradigm. Of those genes we selected candidates for an RNAi screen in which we identified genes causing increased or decreased LTM.
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| Overall design |
Comparative gene profiling of targeted DamID-derived sequencing in Drosophila melanogaster. Triplicates of two types of sample (Dam-CrebB vs Dam-Only) are treated in three different conditions (paired, unpaired and naives).
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| Contributor(s) |
Sgammeglia N, Widmer YF, Kaldun JC, Fritsch C, Bruggmann R, Sprecher SG |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Oct 18, 2022 |
| Last update date |
Oct 21, 2022 |
| Contact name |
Noemi Sgammeglia |
| Organization name |
University of Fribourg
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| Lab |
Sprecher lab
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| Street address |
Chemin du Musee 10
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| City |
Fribourg |
| ZIP/Postal code |
1700 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL23323 |
Illumina HiSeq 3000 (Drosophila melanogaster) |
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| Samples (30)
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| GSM6657715 |
Naives, no training, Dam-Creb, rep1 |
| GSM6657716 |
Naives, no training, Dam-Creb, rep2 |
| GSM6657717 |
Naives, no training, Dam-Creb, rep3 |
| GSM6657718 |
Paired training, Dam-Only, rep1_TI1 |
| GSM6657719 |
Paired training, Dam-Only, rep2_TI1 |
| GSM6657720 |
Paired training, Dam-Only, rep3_TI1 |
| GSM6657721 |
Paired training, Dam-Creb, rep1_TI1 |
| GSM6657722 |
Paired training, Dam-Creb, rep2_TI1 |
| GSM6657723 |
Paired training, Dam-Creb, rep3_TI1 |
| GSM6657724 |
Unpaired training, Dam-Only, rep1_TI1 |
| GSM6657725 |
Unpaired training, Dam-Only, rep2_TI1 |
| GSM6657726 |
Unpaired training, Dam-Only, rep3_TI1 |
| GSM6657727 |
Unpaired training, Dam-Creb, rep1_TI1 |
| GSM6657728 |
Unpaired training, Dam-Creb, rep2_TI1 |
| GSM6657729 |
Unpaired training, Dam-Creb, rep3_TI1 |
| GSM6657730 |
Paired training, Dam-Only, rep1_TI2 |
| GSM6657731 |
Paired training, Dam-Only, rep2_TI2 |
| GSM6657732 |
Paired training, Dam-Only, rep3_TI2 |
| GSM6657733 |
Paired training, Dam-Creb, rep1_TI2 |
| GSM6657734 |
Paired training, Dam-Creb, rep2_TI2 |
| GSM6657735 |
Paired training, Dam-Creb, rep3_TI2 |
| GSM6657736 |
Unpaired training, Dam-Only, rep1_TI2 |
| GSM6657737 |
Unpaired training, Dam-Only, rep2_TI2 |
| GSM6657738 |
Unpaired training, Dam-Only, rep3_TI2 |
| GSM6657739 |
Unpaired training, Dam-Creb, rep1_TI2 |
| GSM6657740 |
Unpaired training, Dam-Creb, rep2_TI2 |
| GSM6657741 |
Unpaired training, Dam-Creb, rep3_TI2 |
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| Relations |
| BioProject |
PRJNA891757 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE216045_NCB1-vs-Dam.gatc.gff.gz |
4.7 Mb |
(ftp)(http) |
GFF |
| GSE216045_NCB2-vs-Dam.gatc.gff.gz |
4.7 Mb |
(ftp)(http) |
GFF |
| GSE216045_NCB3-vs-Dam.gatc.gff.gz |
4.7 Mb |
(ftp)(http) |
GFF |
| GSE216045_PCB1-vs-Dam.gatc.gff.gz |
5.1 Mb |
(ftp)(http) |
GFF |
| GSE216045_PCB11-vs-Dam.gatc.gff.gz |
4.6 Mb |
(ftp)(http) |
GFF |
| GSE216045_PCB12-vs-Dam.gatc.gff.gz |
4.7 Mb |
(ftp)(http) |
GFF |
| GSE216045_PCB13-vs-Dam.gatc.gff.gz |
4.7 Mb |
(ftp)(http) |
GFF |
| GSE216045_PCB2-vs-Dam.gatc.gff.gz |
4.9 Mb |
(ftp)(http) |
GFF |
| GSE216045_PCB3-vs-Dam.gatc.gff.gz |
4.7 Mb |
(ftp)(http) |
GFF |
| GSE216045_UCB1-vs-Dam.gatc.gff.gz |
5.0 Mb |
(ftp)(http) |
GFF |
| GSE216045_UCB11-vs-Dam.gatc.gff.gz |
4.6 Mb |
(ftp)(http) |
GFF |
| GSE216045_UCB12-vs-Dam.gatc.gff.gz |
4.7 Mb |
(ftp)(http) |
GFF |
| GSE216045_UCB13-vs-Dam.gatc.gff.gz |
4.5 Mb |
(ftp)(http) |
GFF |
| GSE216045_UCB2-vs-Dam.gatc.gff.gz |
5.0 Mb |
(ftp)(http) |
GFF |
| GSE216045_UCB3-vs-Dam.gatc.gff.gz |
4.6 Mb |
(ftp)(http) |
GFF |
| GSE216045_compared_PCB11vsUCB11_rep1.bedgraph.gz |
4.8 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE216045_compared_PCB12vsUCB12_rep2.bedgraph.gz |
4.8 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE216045_compared_PCB13vsUCB13_rep3.bedgraph.gz |
4.9 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE216045_compared_PCB1vsUCB1_rep1.bedgraph.gz |
4.9 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE216045_compared_PCB2vsUCB2_rep2.bedgraph.gz |
5.0 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE216045_compared_PCB3vsUCB3_rep3.bedgraph.gz |
4.8 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE216045_filtered_Compared_PCB_t1_results_1_percent_FDR_and_0.2_log_ratio_change_all_transcripts.txt.gz |
3.3 Kb |
(ftp)(http) |
TXT |
| GSE216045_filtered_Compared_PCB_t2_results_1_percent_FDR_and_0.2_log_ratio_change_all_transcripts.txt.gz |
1.2 Kb |
(ftp)(http) |
TXT |
| GSE216045_filtered_Naive_results_1_percent_FDR_and_0.2_log_ratio_change_all_transcripts.txt.gz |
60.3 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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