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| Status |
Public on Dec 28, 2022 |
| Title |
Transcriptome analysis of total RNA from the Rhodobacter sphaeroides 2.4.1 UdsC (sRNA) deletion mutant strain and UdsC (sRNA) overexpression strain under microaerobic growth conditions |
| Organism |
Cereibacter sphaeroides |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The sRNA UdsC ist derived from the 3´ UTR of RSP_7527. To analyse the effect of the sRNA on the transcriptome of R. sphaeroides we performed the RNAseq analysis with a chromosomal knockout and an inducible overexpression of this sRNA.
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| Overall design |
Comparative gene expression profiling analysis of RNAseq data of independent biological triplicates of the Rhodobacter sphaeroides UdsC deletion mutant strain and inducible UdsC overexpression strain under microaerobic growth conditions.
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| Contributor(s) |
Spanka D, Grützner J, Klug G |
| Citation(s) |
36555125 |
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| Submission date |
Oct 19, 2022 |
| Last update date |
Dec 29, 2022 |
| Contact name |
Gabriele Klug |
| E-mail(s) |
Gabriele.Klug@mikro.bio.uni-giessen.de
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| Organization name |
Justus-Liebig University Gießen
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| Department |
Molecular- and Microbiology
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| Street address |
Heinrich-Buff-Ring 26-32
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| City |
Gießen |
| ZIP/Postal code |
35392 |
| Country |
Germany |
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| Platforms (1) |
| GPL32157 |
Illumina NextSeq 500 (Cereibacter sphaeroides) |
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| Samples (9)
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| GSM6658862 |
OE, UdsC, microaerob, 0min IPTG, Rep 1 |
| GSM6658863 |
OE, UdsC, microaerob, 0min IPTG, Rep 2 |
| GSM6658864 |
OE, UdsC, microaerob, 0min IPTG, Rep 3 |
| GSM6658865 |
OE, UdsC, microaerob, 15min IPTG, Rep 1 |
| GSM6658866 |
OE, UdsC, microaerob, 15min IPTG, Rep 2 |
| GSM6658867 |
OE, UdsC, microaerob, 15min IPTG, Rep 3 |
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| Relations |
| BioProject |
PRJNA892101 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE216116_RAW.tar |
102.7 Mb |
(http)(custom) |
TAR (of WIG) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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