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| Status |
Public on Oct 26, 2022 |
| Title |
Functional role of GATA3 and CDX2 in lineage specification during bovine early embryonic development |
| Organism |
Bos taurus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Current understandings of the initiation of the trophectoderm (TE) program in mammalian embryonic development lacks evidence of how TE-associated factors such as CDX2 and GATA3 participate in bovine lineage specification. In this study, we describe the effects of TE-associated factors on lineage specification marker genes such as SOX2, OCT4, NANOG, GATA6 and SOX17, assisted by a cytosine base editor system. Interestingly, GATA3 downregulates the NANOG expression in bovine blastocysts. Further analysis of the blastocyst mosaic shows that GATA3 is required for NANOG in TE cells of bovine blastocysts. It is worthy to notice that, unlike mouse embryos where GATA3 and CDX2 did not reciprocally affect each other in bovine embryos.
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| |
|
| Overall design |
PolyA RNA profiles of bovine embryos
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| Web link |
https://pubmed.ncbi.nlm.nih.gov/36630554/
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| |
|
| Contributor(s) |
Wang H, Shi Y, Hu B |
| Citation(s) |
36630554 |
| Submission date |
Oct 19, 2022 |
| Last update date |
Feb 07, 2023 |
| Contact name |
Kun Zhang |
| E-mail(s) |
kzhang@zju.edu.cn
|
| Organization name |
Zhejiang University
|
| Street address |
866 Yuhangtang Rd
|
| City |
Hangzhou |
| ZIP/Postal code |
310058 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL26012 |
Illumina NovaSeq 6000 (Bos taurus) |
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| Samples (11)
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| Relations |
| BioProject |
PRJNA892118 |
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