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| Status |
Public on Jul 23, 2024 |
| Title |
A single RRM domain-containing protein SARP1 is required for the separation zone establishment in Arabidopsis |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Abscission is a developmental process that sheds organs in response to developmental and environmental cues. Abscission involves cell separation between two neighboring cell types in the abscission zone (AZ), residuum cells (RECs) and secession cells (SECs). However, the regulatory mechanisms for spatial determination governing cell separation are poorly understood. BREVIPEDICELLUS (BP) is one of the key transcription factors regulating the AZ cell size and number. To identify molecular components of abscission, we performed a genetic screen using a weak complementation line of bp-3 carrying activation T-DNA tagging. We identified an ebp1 (enhancer of bp1) mutant displaying delayed floral organ abscission. The ebp1 mutants show concaved surface in SECs and abnormally stacked cells on the top of RECs, in contrast to the precisely separated surface in WT. Molecular and histological analyses revealed that the transcriptional programming during cell differentiation in the AZ is compromised in the ebp1 mutant, where SECs of the ebp1 mutant appear to acquire REC-like activities, including cuticle formation and superoxide production. Here we present that SEPARATION AFFECTING RNA-BINDING PROTEIN1 (SARP1) is up-regulated in ebp1 and plays a role in the establishment of the cell separation layer during floral organ abscission in Arabidopsis.
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| Overall design |
RECs (Residuum cells, abscission zone cells of the receptacle) and SECs (Secession cells, abscission zone cells of separated floral organs) of ebp1 mutants were isolated using fluorescence-activated cell sorting of cells from transgenic plants harboring proQRT2::nlsGFP–GUS construct, and their transcriptomes were analyzed by RNA-sequencing.
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| Contributor(s) |
Yun J, Lee I, Lee J, Kim S, Jung S, Lee J, Park S, Soh M, Lee Y, Kwak JM |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Oct 20, 2022 |
| Last update date |
Jul 24, 2024 |
| Contact name |
Ju Yun |
| E-mail(s) |
hanmak7@naver.com
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| Phone |
82-010-7210-5171
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| Organization name |
DGIST
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| Department |
New Biology
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| Lab |
Lab. of Cell Signaling
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| Street address |
333, Techno jungang-daero
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| City |
Daegu |
| ZIP/Postal code |
42988 |
| Country |
South Korea |
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| Platforms (1) |
| GPL13222 |
Illumina HiSeq 2000 (Arabidopsis thaliana) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA892441 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE216148_RAW.tar |
500.0 Kb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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