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| Status |
Public on Oct 23, 2022 |
| Title |
Comparative transcriptomic and proteomic analysis reveals common molecular factors responsive to heat and drought stress in sweetpotato (Ipomoea batatas) |
| Organism |
Ipomoea batatas |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
In the process of field production, crops are often affected by a variety of abiotic stresses, among which heat (HT) and drought (DR) stress are the most common co-stresses in summer. Although a large number of studies have been carried out on HT and DR stress respectively, little is known about how their combination (DH) affects plants. In this study, we investigated the responses of sweetpotato to HT, DR or DH stress by RNA-seq and DIA technologies, and set up a controlled experiment and quantified gene expression and protein concentrations from paired samples. A total of 12 cDNA libraries were constructed under HT, DR, DH and control condition. We identified 536, 389 and 907 DEGs in response to HT, DR or DH stress, of which 147 were shared and 447 were specifically identified under DH stress. Proteomic analysis identified 1609, 1168 and 1535 DEPs under HT, DR and DH treatments compared to the control, of which 656 were shared and 358 were exclusively identified under DH stress. Further analysis revealed that some DEGs/DEPs associated with heat shock protein, carbon metabolism, phenylalanine metabolism, starch and cellulose metabolism, plant defense and so on. Correlation analysis identified a number of co-expressed genes and proteins under HT, DR or DH stress. Meanwhile, a cross-comparison of transcriptomics and proteomics data identified 59, 35 and 86 significant co DEGs and DEPs genes under HT, DR and DH stress respectively. This is the first time that studies the differential genes and proteins of sweet potato under DH stress, and hopes that the results of this study will help us to understand the molecular mechanism of sweet potato resistance to heat and drought stress.
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| Overall design |
RNA samples from leaves and tuberous roots at each time point under heat, drought and their co-stress respectively as a mixing pool to construct four sequencing libraries, named as HT (heat), DR (drought), DH (drought and heat) and CK (control)
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| Contributor(s) |
Tang W, Arisha MH |
| Citation(s) |
36743565 |
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| Submission date |
Oct 20, 2022 |
| Last update date |
Feb 10, 2023 |
| Contact name |
Wei Tang |
| E-mail(s) |
tangwei2001_20@aliyun.com
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| Organization name |
Xuzhou Institute of Agricultural Sciences in Jiangsu Xuhuai District
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| Street address |
Kunpeng Road num 78
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| City |
Xuzhou |
| ZIP/Postal code |
221131 |
| Country |
China |
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| Platforms (1) |
| GPL22614 |
Illumina HiSeq 2500 (Ipomoea batatas) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA892450 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE216152_RAW.tar |
2.1 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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