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| Status |
Public on Mar 17, 2023 |
| Title |
Induction of fetal meiotic oocytes from embryonic stem cells in cynomolgus monkeys (EM-Seq) |
| Organisms |
Macaca fascicularis; Homo sapiens |
| Experiment type |
Methylation profiling by high throughput sequencing
|
| Summary |
Human in vitro oogenesis provides a framework for clarifying the mechanism of human oogenesis. To create its benchmark, it is vital to promote in vitro oogenesis using a model physiologically close to humans. Here, we establish a foundation for in vitro oogenesis in cynomolgus (cy) monkeys (Macaca fascicularis): cy female embryonic stem cells harboring one active and one inactive X chromosome (Xa and Xi, respectively) differentiate robustly into primordial germ cell-like cells, which in xenogeneic reconstituted ovaries develop efficiently into oogonia and, remarkably, further into meiotic oocytes at the zygotene stage. This differentiation entails comprehensive epigenetic reprogramming, including Xi reprogramming, yet Xa and Xi remain epigenetically asymmetric with, as partly observed in vivo, incomplete Xi reactivation. In humans and monkeys, the Xi epigenome in pluripotent stem cells functions as an Xi-reprogramming determinant. We further show that developmental pathway over-activations with suboptimal up-regulation of relevant meiotic genes impede in vitro meiotic progression. Cy in vitro oogenesis exhibits critical homology with the human system, including with respect to bottlenecks, providing a salient model for advancing human in vitro oogenesis.
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| Overall design |
Whole genome EMseq analysis of Cynomolgus xrOvary and human iPSC
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| |
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| Contributor(s) |
Gyobu-Motani S, Yabuta Y, Mizuta K, Katou Y, Okamoto I, Kawasaki M, Kitamura A, Tsukiyama T, Iwatani C, Tsuchiya H, Tsujimura T, Yamamoto T, Nakamura T, Saitou M |
| Citation(s) |
36929479 |
| |
| Submission date |
Oct 20, 2022 |
| Last update date |
May 07, 2024 |
| Contact name |
Yukihiro Yabuta |
| E-mail(s) |
yabyab@anat2.med.kyoto-u.ac.jp
|
| Organization name |
Kyoto University, Graduate school of medicine
|
| Department |
Anatomy and Cell Biology
|
| Street address |
Yoshida-Konoe-cho, Sakyo-ku
|
| City |
Kyoto |
| State/province |
Kyoto |
| ZIP/Postal code |
606-8501 |
| Country |
Japan |
| |
|
| Platforms (2) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
| GPL28212 |
Illumina NovaSeq 6000 (Macaca fascicularis) |
|
| Samples (13)
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| This SubSeries is part of SuperSeries: |
| GSE216206 |
Induction of fetal meiotic oocytes from embryonic stem cells in cynomolgus monkeys |
|
| Relations |
| BioProject |
PRJNA892580 |
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