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| Status |
Public on May 06, 2010 |
| Title |
Human bone-derived mesenchymal stem cells: SLE patients vs normal controls |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Previous studies showed that bone marrow derived-mesenchymal stem cells (BMMSCs) from patients with systemic lupus erythematosus (SLE) and lupus animal models have deficiency in their capacities of proliferation, differentiation, secretion of cytokines and other functions. In this study, we aim to investigate the different gene patterns of BMMSCs between normal and SLE individuals using genome-scale DNA microarrays. We found that among all the genes investigated in microarray slides, a total of 1, 905 genes were differentially expressed by BMMSCs SLE patients, in which 652 genes were up-regulated and 1253 genes were down-regulated. Gene ontology analysis showed that the majority of these genes were related to cell cycle and protein binding. Pathway analysis showed that differentially regulated signal pathways involved actin cytoskeleton, focal adhesion, tight junction and TGF-β pathway. Our data suggested that there were differential gene expression patterns of BMMSCs between normal and SLE individuals, and further studies are needed to reveal the mechanisms for such differences.
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| Overall design |
Bone marrow (BM) was obtained from 4 patients with SLE and 4 normal controls. BMMSCs were cultured and mRNA was extracted. Standard human reference RNA were purchased. Every slide: SLE or normal vs control, then SLE vs normals.
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| Contributor(s) |
Tang Y, Sun L |
| Citation(s) |
22966240, 22897816 |
| |
| Submission date |
May 04, 2010 |
| Last update date |
Aug 13, 2019 |
| Contact name |
Yu Tang |
| E-mail(s) |
cattlemouse@sohu.com
|
| Phone |
+86-25-8310-5219
|
| Fax |
+86-25-8310-5219
|
| Organization name |
The Affiliated Drum Tower Hospital of Nanjing University Medical School
|
| Department |
Department of Rheumatology and Immunology
|
| Lab |
Laboratory of Rheumatology and Immunology
|
| Street address |
321 Zhongshan Road
|
| City |
Nanjing |
| State/province |
Jiangsu Province |
| ZIP/Postal code |
21008 |
| Country |
China |
| |
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| Platforms (1) |
| GPL10381 |
Nanjing University 22K Human Genome Array |
|
| Samples (8)
|
| GSM540066 |
Normal human bone marrow-derived mesenchymal stem cell 1 |
| GSM540067 |
Normal human bone marrow-derived mesenchymal stem cell 2 |
| GSM540068 |
Normal human bone marrow-derived mesenchymal stem cell 3 |
| GSM540069 |
Normal human bone marrow-derived mesenchymal stem cell 4 |
| GSM540070 |
SLE human bone marrow-derived mesenchymal stem cell 1 |
| GSM540071 |
SLE human bone marrow-derived mesenchymal stem cell 2 |
| GSM540072 |
SLE human bone marrow-derived mesenchymal stem cell 3 |
| GSM540073 |
SLE human bone marrow-derived mesenchymal stem cell 4 |
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| Relations |
| BioProject |
PRJNA125923 |
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