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| Status |
Public on Mar 08, 2024 |
| Title |
Analysing the effect of cellular energy levels on codon-specific ribosome occupancy in vivo |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Other
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| Summary |
The aim of this study is to evaluate how cellular energy levels shape codon-specific decoding and affect codon-mediated mRNA degradation. To this end we made use of HT5P-seq (Zhang & Pelechano, 2021, PMID:35474692) in S. cerevisiae to footprint the ribosome of co-translationally degraded mRNAs in vivo upon swift changes in the concentration of intracellular ATP and other energy metabolites.
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| Overall design |
HT5P-seq was performed in 5 different time points (3 replicates each time points), spanning 5 min before and 10 min after the addition of antimycin A to respiring yeast as described in Walther T et al. (PMID: 20087341)
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| Contributor(s) |
Zhang Y, da Silva P, Gagneur J, Pelechano V |
| Citation(s) |
38491213 |
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| Submission date |
Oct 25, 2022 |
| Last update date |
May 23, 2024 |
| Contact name |
Vicent Pelechano |
| E-mail(s) |
vicente.pelechano.garcia@ki.se
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| Organization name |
ScilifeLab - Karolinska Institutet
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| Department |
MTC
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| Street address |
Nobels väg 16
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| City |
Solna |
| ZIP/Postal code |
SE-17177 |
| Country |
Sweden |
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| Platforms (1) |
| GPL31112 |
NextSeq 2000 (Saccharomyces cerevisiae) |
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| Samples (15)
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| Relations |
| BioProject |
PRJNA894167 |
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