|
| Status |
Public on Dec 19, 2022 |
| Title |
Augmenting hematoma-scavenging capacity of innate immune cells by CDNF reduces brain injury and promotes functional recovery after intracerebral hemorrhage |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We show that intracerebroventricular administration of recombinant human cerebral dopamine neurotrophic factor (rhCDNF) accelerates hemorrhagic lesion resolution, reduces peri-focal edema, and improves neurological outcomes in an animal model of collagenase-induced ICH. We demonstrate that CDNF acts on microglia/macrophages in the hemorrhagic striatum by promoting scavenger receptor expression, enhancing erythrophagocytosis and increasing anti-inflammatory mediators while suppressing the production of pro-inflammatory cytokines. Administration of rhCDNF results in upregulation of the Nrf2-HO-1 pathway, but alleviation of oxidative stress and unfolded protein responses in the perihematomal area. Finally, we demonstrate that intravenous delivery of rhCDNF has beneficial effects in an animal model of ICH and that systemic application promotes scavenging by the brain’s myeloid cells for the treatment of ICH.
|
| |
|
| Overall design |
Comparison of CD11+positive cells extracted from hemorrhagic striatum of rhCDNF or saline treated animals
|
| |
|
| Contributor(s) |
Kuan-Yin T, Stratoulias V, Airavaara M, Väänänen J, Hock-Kean L |
| Citation(s) |
36792604 |
| Submission date |
Oct 25, 2022 |
| Last update date |
Mar 20, 2023 |
| Contact name |
Vassilis Stratoulias |
| E-mail(s) |
vassileios.stratoulias@helsinki.fi
|
| Phone |
+358505962498
|
| Organization name |
University of Helsinki
|
| Street address |
Haartmaninkatu 8
|
| City |
Helsinki |
| State/province |
<None> |
| ZIP/Postal code |
00014 |
| Country |
Finland |
| |
|
| Platforms (1) |
| GPL20084 |
Illumina NextSeq 500 (Rattus norvegicus) |
|
| Samples (10)
|
|
| Relations |
| BioProject |
PRJNA894196 |
Less...
More...