|
|
GEO help: Mouse over screen elements for information. |
|
Status |
Public on Mar 04, 2024 |
Title |
H2A.Z is required to initiate DNA repair to prevent premature aging |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
|
Summary |
Histone variants are key epigenetic players, but their functional and physiological roles remain poorly understood. Here, we show that depletion of the histone variant H2A.Z in mouse skeletal muscle causes oxidative stress, oxidation of proteins, accumulation of DNA damages, and both neuromuscular junction and mitochondria lesions that consequently lead to premature muscle aging and reduced life span. Investigation of the molecular mechanisms involved shows that H2A.Z is required to initiate DNA double strand break repair by recruiting Ku80 at DNA lesions. This is achieved via specific interactions of Ku80 vWA domain with H2A.Z. Taken as a whole, our data reveal that H2A.Z containing nucleosomes act as a molecular platform to bring together the proteins required to initiate and process DNA double strand break repair.
|
|
|
Overall design |
To adress the effect of H2A.Z on transcription, we have used skeletal muscles isolated from WT and H2A.Z null 12 months-old animals
|
|
|
Contributor(s) |
Belotti E, Lacoste N, Iftikhar A, Simonet T, Papin C, Streichenberger N, Mari P, Girard E, Osseni A, Giglia-Mari G, Dimitrov S, Hamiche A, Schaeffer L |
Citation(s) |
38281187 |
|
Submission date |
Oct 31, 2022 |
Last update date |
May 01, 2024 |
Contact name |
Christophe Papin |
E-mail(s) |
papin@igbmc.fr
|
Organization name |
IGBMC - CNRS UMR 7104 - Inserm U 964
|
Street address |
1 rue Laurent Fries
|
City |
ILLKIRCH-GRAFFENSTADEN |
ZIP/Postal code |
67404 |
Country |
France |
|
|
Platforms (1) |
GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
|
Samples (6)
|
|
Relations |
BioProject |
PRJNA896205 |
Supplementary file |
Size |
Download |
File type/resource |
GSE216935_RNA.xlsx |
4.8 Mb |
(ftp)(http) |
XLSX |
SRA Run Selector |
Raw data are available in SRA |
Processed data are available on Series record |
|
|
|
|
|