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| Status |
Public on May 03, 2023 |
| Title |
Obesity and dyslipidemia are associated with partially reversible modifications to DNA hydroxymethylation in swine adipose-derived mesenchymal stem/stromal cells [Sus scrofa] |
| Organism |
Sus scrofa |
| Experiment type |
Methylation profiling by high throughput sequencing
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| Summary |
Female domestic pigs were fed a 16-week Lean or Obese diet. Mesenchymal stem/stromal cells (MSCs) were harvested from subcutaneous adipose tissue and expanded for 3-4 passages, and 5hmC profiles were examined through hydroxymethylated DNA immunoprecipitation sequencing (hMeDIP-seq)
We hypothesized that obesity and cardiovascular risk factors induce functionally-relevant, locus-specific changes in overall exonic coverage of 5hmC in swine adipose-derived MSCs, and evaluated their reversibility using an epigenetic modulator, vitamin-C.
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| Overall design |
Lean-MSCs and Obese-MSCs were harvested from swine fed a Lean or Obese diet, respectively (n=3, each), and Obese-MSCs were additionally co-incubated with vitamin-C in-vitro
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| Contributor(s) |
Lerman LO, Eirin A, Glasstetter LM, Oderinde TS, Rajagopalan KS, Thaler R |
| Citation(s) |
37231414 |
| |
| Submission date |
Oct 31, 2022 |
| Last update date |
Aug 02, 2023 |
| Contact name |
Lilach O O Lerman |
| E-mail(s) |
lerman.lilach@mayo.edu
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| Phone |
5072545377
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| Organization name |
Mayo Clinic
|
| Department |
Division of Nephrology & Hypertension
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| Street address |
200 First St. SW
|
| City |
Rochester |
| State/province |
Minnesota |
| ZIP/Postal code |
55905 |
| Country |
USA |
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| Platforms (1) |
| GPL22475 |
Illumina HiSeq 4000 (Sus scrofa) |
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| Samples (9)
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| This SubSeries is part of SuperSeries: |
| GSE216953 |
Obesity and dyslipidemia are associated with partially reversible modifications to DNA hydroxymethylation of apoptosis- and senescence-related genes in swine adipose-derived mesenchymal stem/stromal cells |
|
| Relations |
| BioProject |
PRJNA896233 |
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