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| Status |
Public on Nov 09, 2022 |
| Title |
Differential expression and correlation analysis of miRNA–mRNA profiles in longissimus dorsi of different stage goats |
| Organism |
Capra hircus |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
MicroRNAs (miRNAs) are small non-coding RNAs that regulate the post transcriptional control of several pathway intermediates, and essential for regulation in skeletal muscle of many species, such as mice, cattle, pig and so on. However, a little number of miRNAs have been reported in the muscle development of goat. In this study, the longissimus dorsi transcripts of goat at 1- and 10-month-old were analyzed for RNA-seq and miRNA-seq. The results showed that 10-month-old Longlin goat expressed 327 up- and 419 down-regulated differentially expressed genes (DEGs) compared with the 1-month-old were founded. In addition, 20 co-up-regulated and 55 co-down-regulated miRNAs involved in muscle fiber hypertrophy of goat were identified in 10-month-old Longlin and Nubian goat compared with 1-month-old. Five miRNA–mRNA pairs (chi-let-7b-3p-MIRLET7A, chi-miR193b-3p-MMP14, chi-miR-355-5p-DGAT2, novel_128-LOC102178119, novel_140-SOD3) involved in the goat skeletal muscle development were identified by miRNA–mRNA negative correlation network analysis. Our results provided an insight into the functional roles of miRNAs of goat muscle-associated miRNAs, allowing us to better understand the transformation of miRNA roles during mammalian muscle development.
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| Overall design |
MicroRNAs (miRNAs) are small non-coding RNAs that regulate the post transcriptional control of several pathway intermediates, and essential for regulation in skeletal muscle of many species, such as mice, cattle, pig and so on. However, a little number of miRNAs have been reported in the muscle development of goat. In this study, the longissimus dorsi transcripts of goat at 1- and 10-month-old were analyzed for RNA-seq and miRNA-seq. The results showed that 10-month-old Longlin goat expressed 327 up- and 419 down-regulated differentially expressed genes (DEGs) compared with the 1-month-old were founded. In addition, 20 co-up-regulated and 55 co-down-regulated miRNAs involved in muscle fiber hypertrophy of goat were identified in 10-month-old Longlin and Nubian goat compared with 1-month-old. Five miRNA–mRNA pairs (chi-let-7b-3p-MIRLET7A, chi-miR193b-3p-MMP14, chi-miR-355-5p-DGAT2, novel_128-LOC102178119, novel_140-SOD3) involved in the goat skeletal muscle development were identified by miRNA–mRNA negative correlation network analysis. Our results provided an insight into the functional roles of miRNAs of goat muscle-associated miRNAs, allowing us to better understand the transformation of miRNA roles during mammalian muscle development.
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| Contributor(s) |
Jiang Q, Jiang M, Zhang S, Zhang Q |
| Citation(s) |
36833242 |
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| Submission date |
Nov 02, 2022 |
| Last update date |
Mar 09, 2023 |
| Contact name |
Sanbao Zhang |
| E-mail(s) |
gxuzsb@126.com
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| Phone |
18447055384
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| Organization name |
Guangxi University
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| Street address |
No.100,Daxue East Road
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| City |
Nanning |
| ZIP/Postal code |
530004 |
| Country |
China |
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| Platforms (1) |
| GPL15473 |
Illumina HiSeq 2000 (Capra hircus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA896926 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE217092_10-month-old_Longlin_goat_vs_1-month-old_Longlin_goat_DEG_GO_enrichment_result.txt.gz |
863.3 Kb |
(ftp)(http) |
TXT |
| GSE217092_10-month-old_Longlin_goat_vs_1-month-old_Longlin_goat_Differential_analysis_results.txt.gz |
12.9 Kb |
(ftp)(http) |
TXT |
| GSE217092_10-month-old_Nubian_goat_vs_1-month-old_Nubian_goat_.DEG_GO_enrichment_result.txt.gz |
653.5 Kb |
(ftp)(http) |
TXT |
| GSE217092_10-month-old_Nubian_goat_vs_1-month-old_Nubian_goat_Differential_analysis_results.txt.gz |
12.2 Kb |
(ftp)(http) |
TXT |
| GSE217092_Readcount_TPM.txt.gz |
33.2 Kb |
(ftp)(http) |
TXT |
| GSE217092_commom_target.txt.gz |
22.3 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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