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Series GSE21721

Query DataSets for GSE21721

Status

Public on Aug 09, 2012

Title

Transcriptome profiling analysis of bacterial challenged Lateolabrax japonicus reveals deep insight into the immune-relevant genes in marine fish (RNA-seq)

Organism

Lateolabrax japonicus

Experiment type

Expression profiling by high throughput sequencing

Summary

We report the application of Solexa/Illumina’s RNA-seq sequencing approaches for transcriptome in a marine fish under different conditions (bacterial- and mock-challenged conditions). By obtaining over four billion bases of sequence from the cDNA, we generated 169,950 none-redundant consensus sequences, from which 44842 functional transcripts with complete or various length of encoding regions were identified. More than 52% of these transcripts could be enriched in approximately 219 known metabolic or signaling pathways, among of which 2673 transcripts were found to be associated with immune-relevant genes. Besides, about 8% of the transcripts seemed fish-specific genes that have never been described before. This study provides a framework for the application of comprehensive chromatin profiling towards characterization of diverse mammalian cell populations. Our study provided a global survey of the gene activities in host defense against bacterial infection in a non-model marine fish.

Overall design

Examination of different transcriptome in baterial- and mock challenged fish.

Contributor(s)

Shao J, Xiang L, He D, Dong W, Zhang Y

Citation(s)

20707909

Submission date

May 06, 2010

Last update date

May 15, 2019

Contact name

Ding He