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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Mar 10, 2023 |
| Title |
Gene expression profile at single cell level of phagocytic myeloid cells in autochthonous lung cancer model |
| Organism |
Mus musculus |
| Experiment type |
Other
Expression profiling by high throughput sequencing
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| Summary |
Phagocytosis is a critical process undertaken by myeloid cells in the tumor microenvironment. We used single-cell RNA-seq (scRNAseq) to investigate molecular and cellular consequences of neoplastic cell engulfment using a tdTomato genetically engineered mouse model.
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| Overall design |
CD45-positive and CD45-negative populations from the lung tissue of SPC-creER KRAS-G12D mice were isolated by Fluorescence-activated cell sorting (FACS) analyzed using scRNAseq with antibody-based cell hashing.
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| Contributor(s) |
Lu DR, Gonzalez MA, Arias V, Li C, Tarbell KV, Winslow M |
| Citation(s) |
36995340 |
| Submission date |
Nov 05, 2022 |
| Last update date |
Jun 09, 2023 |
| Contact name |
Daniel R Lu |
| Organization name |
Amgen
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| Street address |
750 Gateway Blvd, Suite 100
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| City |
South San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94080 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (20)
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| GSM6716128 |
tdTom-neg myeloid cells from tumor-bearing mice, rep 1, mRNA-derived cDNA |
| GSM6716129 |
tdTom-neg myeloid cells from tumor-bearing mice, rep 1, HTO-derived cDNA |
| GSM6716130 |
tdTom-neg myeloid cells from tumor-bearing mice, rep 2, mRNA-derived cDNA |
| GSM6716131 |
tdTom-neg myeloid cells from tumor-bearing mice, rep 2, HTO-derived cDNA |
| GSM6716132 |
tdTom-pos myeloid cells from tumor-bearing mice, rep 1, mRNA-derived cDNA |
| GSM6716133 |
tdTom-pos myeloid cells from tumor-bearing mice, rep 1, HTO-derived cDNA |
| GSM6716134 |
tdTom-pos myeloid cells from tumor-bearing mice, rep 2, mRNA-derived cDNA |
| GSM6716135 |
tdTom-pos myeloid cells from tumor-bearing mice, rep 2, HTO-derived cDNA |
| GSM6716136 |
tdTom-pos neoplastic cells from tumor-bearing mice, mRNA-derived cDNA |
| GSM6716137 |
tdTom-pos neoplastic cells from tumor-bearing mice, HTO-derived cDNA |
| GSM6716138 |
tdTom-neg non-immune cells from tumor-bearing mice, mRNA-derived cDNA |
| GSM6716139 |
tdTom-neg non-immune cells from tumor-bearing mice, HTO-derived cDNA |
| GSM6716140 |
Non-immune cells from naïve mice, rep 1, mRNA-derived cDNA |
| GSM6716141 |
Non-immune cells from naïve mice, rep 1, HTO-derived cDNA |
| GSM6716142 |
Non-immune cells from naïve mice, rep 2, mRNA-derived cDNA |
| GSM6716143 |
Non-immune cells from naïve mice, rep 2, HTO-derived cDNA |
| GSM6716144 |
Myeloid cells from naïve mice, rep 1, mRNA-derived cDNA |
| GSM6716145 |
Myeloid cells from naïve mice, rep 1, HTO-derived cDNA |
| GSM6716146 |
Myeloid cells from naïve mice, rep 2, mRNA-derived cDNA |
| GSM6716147 |
Myeloid cells from naïve mice, rep 2, HTO-derived cDNA |
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| Relations |
| BioProject |
PRJNA898629 |
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