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| Status |
Public on Nov 21, 2022 |
| Title |
Sex-determining 3D regulatory hubs revealed by genome spatial auto-correlation analysis [RNA-seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Mammalian sex is determined by a network of antagonistic genes that are well characterized. However, its non-coding regulation is still largely unknown. Here we explore the 3D chromatin landscape of sex determination in vivo. We integrate Hi-C with ChIP-seq experiments using METALoci, a novel genome spatial auto-correlation analysis approach that identifies 3D regulatory hubs across the genome. We uncover a prominent rewiring of chromatin interactions during sex determination. By combining predictive approaches and validations in transgenic mice we identify a novel Fgf9 regulatory hub, which deletion results in male-to-female sex reversal.
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| Overall design |
RNA-seq experiments in E13.5 XY WT and E13.5 XY Fgf9 Δ306 mouse gonads.
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| Contributor(s) |
Mota-Gomez I, Rodriguez JA, Dupont S, Lao O, Jedamzick J, Kühn R, Lacadie S, Garcia-Moreno SA, Hurtado A, Acemel RD, Capel B, Marti-Renom MA, Lupianez DG |
| Citation missing |
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| |
| Submission date |
Nov 09, 2022 |
| Last update date |
Nov 29, 2022 |
| Contact name |
Irene Mota-Gomez |
| E-mail(s) |
irene211092@gmail.com
|
| Organization name |
Max Delbruck Center
|
| Lab |
Dario Lupianez
|
| Street address |
Hannoversche strasse 28
|
| City |
Berlin |
| ZIP/Postal code |
10115 |
| Country |
Germany |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (6)
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| GSM6722715 |
RNA-seq in XY E13.5 Fgf9 Δ306 gonads rep1 |
| GSM6722716 |
RNA-seq in XY E13.5 Fgf9 Δ306 gonads rep2 |
| GSM6722717 |
RNA-seq in XY E13.5 Fgf9 Δ306 gonads rep3 |
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| This SubSeries is part of SuperSeries: |
| GSE217618 |
Sex-determining 3D regulatory hubs revealed by genome spatial auto-correlation analysis |
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| Relations |
| BioProject |
PRJNA899837 |
| SRA |
SRP407014 |
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