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| Status |
Public on Feb 13, 2023 |
| Title |
OmpC and bile tolerance are important for Escherichia coli colonization of the mammalian intestine |
| Organism |
Escherichia coli str. K-12 substr. MG1655 |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Our focus of this study is to determine the gene expression changes in E. coli colonizing the mouse intestine as compared to E. coli grown in minimal medium in vitro. We colonized CD-1 male mice with E. coli MG1655 StrR wild type and extracted the total RNA from mouse cecal mucus. We also extracted E. coli MG1655 StrR RNA from in vitro culture and sequenced and compared to determine the genes important for E. coli colonization of the mammalian intestine. Our results indicate that OmpC is important for E. coli to colonize the mouse intestine as it is upregulated as compared to in vitro culture. OmpF is not important; it is detrimental for E. coli colonization of the mouse intestine and is highly downregulated in the colonized E. coli
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| Overall design |
E. coli MG1655 StrR was fed to CD-1 male mice and on the 9th day, the mice were euthanized, mucus from the cecum was obtained and total RNA was extracted and sequenced. E. coli MG1655 StrR was cultured in MOPS minimal glucose medium, and the total transcriptome of exponential phase culture was sequenced
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| Contributor(s) |
Doranga S, Conway T |
| Citation(s) |
37014216, 38380942 |
| |
| Submission date |
Nov 10, 2022 |
| Last update date |
Mar 20, 2024 |
| Contact name |
Tyrrell Conway |
| Organization name |
Oklahoma state university
|
| Department |
Microbiology and Molecular Genetics
|
| Lab |
Conway lab
|
| Street address |
307 Life sciences east, OKLAHOMA STATE UNIVERSITY
|
| City |
STILLWATER |
| State/province |
OKLAHOMA |
| ZIP/Postal code |
74078 |
| Country |
USA |
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| Platforms (1) |
| GPL26592 |
Illumina NovaSeq 6000 (Escherichia coli str. K-12 substr. MG1655) |
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| Samples (5)
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| Relations |
| BioProject |
PRJNA900253 |
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