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| Status |
Public on Jul 15, 2024 |
| Title |
Fosl2 overexpression and Ezh2 inhibition in mouse embryonic fibroblasts |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
To assess the impact of AP-1 chromatin remodelling, we over-expressed one AP-1 family member, namely Fra2 (encoded by Fosl2), in mouse embryonic fibroblasts (MEFs).
To assess the impact of general chromatin depression, we inhibited the activity of PRC2 component histone-lysine N-methyltransferase Ezh2 in MEFS.
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| Overall design |
Fosl2 overexpression: We transduced mouse embryonic fibroblasts (MEFs) with a CRISPRa-VPR-mCherry construct and assessed more immediate and longer term effects of Fra2 over-expression by performing OMNI-ATACseq on FACS purified mCherry+/THY1+ cells 4 and 11 days following lentiviral infection with Fra2 gRNAs or scrambled control.
For Ezh2 inbition: Following three days of treatment with the Ezh2 inhibitor (Sigma-Aldrich, GSK343) at 4microM and a washout day, MEFs were submitted to the ATAC-seq assay.
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| Contributor(s) |
Nefzger C, Zhang J, Chen X, Patrick R, Naval-Sanchez M |
| Citation(s) |
38959897 |
| |
| Submission date |
Nov 11, 2022 |
| Last update date |
Jul 16, 2024 |
| Contact name |
Christian Nefzger |
| Organization name |
The University of Queensland
|
| Department |
Institute for Molecular Biology
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| Lab |
Cellular Reprogramming and Ageing
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| Street address |
306 Carmody Road
|
| City |
Brisbane |
| State/province |
Queensland |
| ZIP/Postal code |
4067 |
| Country |
Australia |
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| Platforms (1) |
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| Samples (15)
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| Relations |
| BioProject |
PRJNA900535 |
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