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| Status |
Public on Sep 27, 2023 |
| Title |
Malaria drives unique regulatory responses across multiple immune cells during human infection |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To investigate malaria driven transcriptional changes in specific immune cell subsets, we sorted live PBMCs from 6 malaria infected donors (day 0), and two subsequent time points after drug treatment (day 7 and 28), along with PBMCs from 2 healthy controls. We performed scRNAseq of these cells, and used clustering and sub-clustering to identify specific immune cell subsets. Differential gene analysis between day 0 and day 28 was performed for each cell cluster and sub-cluster. Key transcriptional changes were confirmed at the protein level with additional donor samples.
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| Overall design |
Peripheral blood mononuclear cells (PBMCs) from 6 individuals (age 6-24) with P. falciparum infection and 2 healthy adult endemic controls (ages 20 and 27) were collected before drug treatment (day 0) and at 7 and 28 days after treatment. PBMCs were isolated by Fluorescence-activated cell sorting (FACS) according to the presence or absence of XX signal, and analysed using droplet-based scRNAseq.”
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| Contributor(s) |
Boyle M, Dooley N, Chabikwa T, Loughland JR, Pava Z, Hamelink J, Berry K, Andrew D, Soon M, SheelaNair A, Piera K, Williams T, Barber B, Grigg M, Engweda CR, Lopez A, Anstey N |
| Citation(s) |
37968278 |
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| Submission date |
Nov 14, 2022 |
| Last update date |
Dec 07, 2023 |
| Contact name |
Zuleima Pava Imitola |
| Organization name |
QIMR Berghofer
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| Street address |
300 Herston road
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| City |
Brisbane |
| State/province |
Queensland |
| ZIP/Postal code |
4006 |
| Country |
Australia |
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| Platforms (1) |
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| Samples (20)
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| Relations |
| BioProject |
PRJNA901418 |
| SRA |
SRP407776 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE217930_RAW.tar |
683.8 Mb |
(http)(custom) |
TAR (of MTX, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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