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Series GSE218383 Query DataSets for GSE218383
Status Public on Sep 22, 2023
Title Nanostring gene expression data, comparison of response to subcutaneously implanted porcine-derived decellularized myocardial matrix hydrogels in mast cell deficient mice compared to deficient mice with mast cell engraftment and wild-type C57BL6/J mice
Organism Mus musculus
Experiment type Expression profiling by array
Summary Nanostring gene expression count data from the Nanostring Mouse Immunology Panel consisting of 546 genes with 15 internal controls from excised subcutaneous injections of porcine derived decellularized myocardial matrix hydrogels that were lysed and extracted for total RNA. Data was then exported through the manufacturer nSolverTM Analysis Software 4.0 and analyzed through custom R scripts under R versions 4.04 with normalized counts determined by the NanostringDiff package and thresholding outside of one standard deivation from the mean signal from the negative control probes.
 
Overall design In this study, porcine-derived decellularized myocardial matrix hydrogels were injected into the dorsal region of male 11-13 week old mast cell deficient mice (w-sh mice: B6.Cg-KitW-sh/HNihrJaeBsmJ, Jackson Laboratory, Sacramento, CA), mast cell engrafted mice and wild-type C57BL6/J. Hydrogels were isolated at 11 days post-injection based on latest timepoint for discernible presence hydrogel degradation profile and analyzed for gene expression analysis by Nanostring Mouse Immunology Panel. Mast cell-engrafted mice were created by delivering eight to ten 100 ┬ÁL subcutaneous injections of bone marrow differentiated mast cells in DMEM totaling 4 million cells. Injections were at evenly spaced intervals throughout the dorsal region of 4-6 week old deficient mice.
 
Contributor(s) Wang RM, Christman KL
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Submission date Nov 19, 2022
Last update date Sep 22, 2023
Contact name Raymond Ma Wang
E-mail(s) ruimawang@gmail.com
Organization name University of California San Diego
Department Bioengineering
Lab Sanford Consortium for Regenerative Medicine
Street address 2880 Torrey Pines Scenic Dr
City La Jolla
State/province California
ZIP/Postal code 92037
Country USA
 
Platforms (1)
GPL30438 nCounter Mouse Immunology Panel [NS_Immunology_Mm_C2269]
Samples (24)
GSM6743998 Wild-type 1
GSM6743999 Wild-type 2
GSM6744000 Wild-type 3
Relations
BioProject PRJNA903402

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE218383_RAW.tar 170.0 Kb (http)(custom) TAR (of RCC)
Processed data included within Sample table

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