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Series GSE218660 Query DataSets for GSE218660
Status Public on Sep 06, 2023
Title Inflammation alters young and old CD8 T cells
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Inflammation plays a significant role in lung infection caused by Mycobacterium tuberculosis (M.tb), and both adaptive and innate lymphocytes can affect infection control. How inflammation affects infection is understood in a broad sense, including inflammaging (chronic inflammation) seen in the elderly, but the explicit role that inflammation can play in regulation of lymphocyte function is not known. To fill this knowledge gap, we used an acute lipopolysaccharide (LPS) treatment in young mice and studied lymphocyte responses compared to old mice, focusing on CD8 T cell subsets. LPS treatment decreased the total numbers of T cells in the lungs of LPS mice, while also increasing the number of activated T cells. We demonstrate that lung CD8 T cells from LPS mice became capable of an antigen independent innate-like IFN-gamma secretion, dependent on IL-12p70 stimulation, paralleling innate-like IFN-gamma secretion of lung CD8 T cells from old mice. Overall, this study provides information on how acute inflammation can affect lymphocytes, particularly CD8 T cells, which could potentially affect immune control of various disease states.
Overall design RNA-seq analysis was performed on CD8 T cells isolated from the lungs of LPS treated, young, and old mice (4 replicates/group).
Contributor(s) Piergallini TJ, Scordo JM, Allué-Guardia A, Pino PA, Zhang H, Cai H, Wang Y, Schlesinger LS, Torrelles JB, Turner J
Citation(s) 37196159
Submission date Nov 23, 2022
Last update date Sep 06, 2023
Contact name Anna Allue Guardia
Organization name Texas Biomedical Research Institute
Street address 8715 Military Dr W
City San Antonio
State/province Texas
ZIP/Postal code 78227
Country USA
Platforms (1)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (12)
GSM6754185 CD8 T cells, LPS, rep1
GSM6754186 CD8 T cells, LPS, rep2
GSM6754187 CD8 T cells, LPS, rep3
BioProject PRJNA904766

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Supplementary file Size Download File type/resource
GSE218660_Turner_CD8_Raw.txt.gz 448.4 Kb (ftp)(http) TXT
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