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| Status |
Public on Sep 06, 2023 |
| Title |
Inflammation alters young and old CD8 T cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Inflammation plays a significant role in lung infection caused by Mycobacterium tuberculosis (M.tb), and both adaptive and innate lymphocytes can affect infection control. How inflammation affects infection is understood in a broad sense, including inflammaging (chronic inflammation) seen in the elderly, but the explicit role that inflammation can play in regulation of lymphocyte function is not known. To fill this knowledge gap, we used an acute lipopolysaccharide (LPS) treatment in young mice and studied lymphocyte responses compared to old mice, focusing on CD8 T cell subsets. LPS treatment decreased the total numbers of T cells in the lungs of LPS mice, while also increasing the number of activated T cells. We demonstrate that lung CD8 T cells from LPS mice became capable of an antigen independent innate-like IFN-gamma secretion, dependent on IL-12p70 stimulation, paralleling innate-like IFN-gamma secretion of lung CD8 T cells from old mice. Overall, this study provides information on how acute inflammation can affect lymphocytes, particularly CD8 T cells, which could potentially affect immune control of various disease states.
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| Overall design |
RNA-seq analysis was performed on CD8 T cells isolated from the lungs of LPS treated, young, and old mice (4 replicates/group).
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| Contributor(s) |
Piergallini TJ, Scordo JM, Allué-Guardia A, Pino PA, Zhang H, Cai H, Wang Y, Schlesinger LS, Torrelles JB, Turner J |
| Citation(s) |
37196159 |
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| Submission date |
Nov 23, 2022 |
| Last update date |
Sep 06, 2023 |
| Contact name |
Anna Allue Guardia |
| Organization name |
Texas Biomedical Research Institute
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| Street address |
8715 Military Dr W
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| City |
San Antonio |
| State/province |
Texas |
| ZIP/Postal code |
78227 |
| Country |
USA |
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| Platforms (1) |
| GPL21493 |
Illumina HiSeq 3000 (Mus musculus) |
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| Samples (12)
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| GSM6754188 |
CD8 T cells, LPS, rep4 |
| GSM6754189 |
CD8 T cells, Old, rep1 |
| GSM6754190 |
CD8 T cells, Old, rep2 |
| GSM6754191 |
CD8 T cells, Old, rep3 |
| GSM6754192 |
CD8 T cells, Old, rep4 |
| GSM6754193 |
CD8 T cells, Young, rep1 |
| GSM6754194 |
CD8 T cells, Young, rep2 |
| GSM6754195 |
CD8 T cells, Young, rep3 |
| GSM6754196 |
CD8 T cells, Young, rep4 |
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| Relations |
| BioProject |
PRJNA904766 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE218660_Turner_CD8_Raw.txt.gz |
448.4 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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