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| Status |
Public on Dec 01, 2022 |
| Title |
The m6A methyltransferase METTL3 participate in the attenuated effect of BPA on learning and memory in rat |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
Methylation profiling by high throughput sequencing
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| Summary |
Bisphenol A (BPA), a widely used endocrine disruptor, has been implicated in cognitive impairment via epigenetic machinery. N6-methyladenosine (m6A) has recently emerged as a new epigenetic factor that negatively influencing cognition, but the role of m6A in BPA induced cognitive deficits has not been explored yet.Here, m6A sequencing in total RNA of neurons after BPA exposure was performed and compare.
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| Overall design |
Primary neurons were cultured and exposed to BPA with 1mM for 48h on DIV 12-14. Total RNA was extracted using RNA isolater Total RNA Extraction Reagent (R401-01, Vazyme, China) according to the manufacturer’s instructions. m6A RNA-Seq service was provided by Cloudseq Biotech Inc. (Shanghai, China).
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| Contributor(s) |
Li D, Ai S, Wang H |
| Citation missing |
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| Submission date |
Nov 23, 2022 |
| Last update date |
Dec 03, 2022 |
| Contact name |
Danyang Li |
| E-mail(s) |
celllee@126.com
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| Phone |
13306720723
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| Organization name |
Hefei university of technology
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| Street address |
No. 193 Tunxi Road
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| City |
Hefei |
| ZIP/Postal code |
230009 |
| Country |
China |
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| Platforms (1) |
| GPL25947 |
Illumina NovaSeq 6000 (Rattus norvegicus) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA904811 |
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