|
| Status |
Public on Jul 23, 2010 |
| Title |
Sheep dendritic cells CD26+ and CD26- versus total dendritic cells |
| Organism |
Ovis aries |
| Experiment type |
Expression profiling by array
|
| Summary |
Transcriptomic profiling of ovine skin lymph dendritic cell subsets CD26+ and CD26- compared to total enriched lymph dendritic cells.
CD26+ and CD26- dendritic cells (DC) from sheep skin lymph were sorted by flow cytometry and were analysed for their transcriptomic profile. We demonstrate that the minor sheep CD26+ skin lymph DC subset shares significant transcriptomic similarities with mouse CD8a+ and human BDCA3+ DC. This allowed the identification of a common set of phenotypic characteristics for CD8a-like DC in the 3 mammalian species, i.e. SIRPlo, CADM1hi, CLEC9Ahi, DEC205hi, XCR1hi. Compared to CD26- DC, the sheep CD26+ DC show (1) potent stimulation of allogeneic naive CD8+ T cells with high selective induction of the Ifn-gamma and Il22 genes; (2) dominant efficacy in activating specific CD8+ T cells against exogenous soluble antigen; (3) selective expression of functional pathways associated to high capacity for antigen cross-presentation.
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| |
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| Overall design |
Reference design (total enriched lymph dendritic cells) - Biological replicates: 2 sheep #10081 and #30066
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| |
|
| Contributor(s) |
Schwartz-Cornil I, Dalod M |
| Citation(s) |
20702727 |
| |
| Submission date |
May 18, 2010 |
| Last update date |
Jan 16, 2014 |
| Contact name |
Doulaye Dembele |
| E-mail(s) |
doulaye@igbmc.fr
|
| Phone |
+33 3 88 65 35 28
|
| Organization name |
IGBMC
|
| Department |
Biopuces
|
| Street address |
1 rue Laurent Fries
|
| City |
Illkirch |
| ZIP/Postal code |
67400 |
| Country |
France |
| |
|
| Platforms (1) |
| GPL10427 |
Agilent-019921 Sheep Gene Expression Microarray (Feature Number version) |
|
| Samples (5)
|
|
| Relations |
| BioProject |
PRJNA126901 |
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