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| Status |
Public on Nov 28, 2023 |
| Title |
Influence of Sox4 on transcriptomic changes in adult hepatocytes |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Recent studies have demonstrated that hepatocytes can be reprogrammed into biliary epithelial cell-like cells. An earlier work in our laboratory nominated Sox4 as an initiation factor of this reprogramming event. To investigate the effect of Sox4 on the transcriptomic changes in hepatocytes, we performed RNA-seq of Sox4-overexpressed (OE) hepatocytes.
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| Overall design |
Mice were injected with adeno-associated viruses (AAVs) packaged with mouse Sox4 (AAV8-TBG-HA-Sox4-P2A-Cre) or empty vector (AAV8-TBG-Cre). Hepatocytes were harvested 4 days post injection by a standard 2-step liberase digestion method followed by a Percoll enrichment step.
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| Contributor(s) |
Katsuda T, Sussman J, Stanger BZ |
| Citation(s) |
38409161 |
| |
| Submission date |
Nov 28, 2022 |
| Last update date |
Mar 11, 2024 |
| Contact name |
Takeshi Katsuda |
| E-mail(s) |
tkatsuda412@gmail.com
|
| Phone |
+1-215-746-5559
|
| Organization name |
University of Pennsylvania
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| Department |
Perelman School of Medicine
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| Lab |
Gastroenterology Division
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| Street address |
421 Curie Blvd, BRB II/III Rm531
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| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
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| Relations |
| BioProject |
PRJNA906174 |
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