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| Status |
Public on Jul 27, 2023 |
| Title |
Intradermal but not intramuscular MVA immunizations protect against intravaginal tier2 SHIV challenges in macaques |
| Organism |
Macaca mulatta |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Route of immunization can markedly influence the quality of immune response. Here, we show that intradermal (ID) but not intramuscular (IM) modified vaccinia Ankara (MVA) vaccinations provide protection from acquisition of intravaginal tier2 SHIV challenges in female macaques. Both routes of vaccination induced comparable levels of serum IgG with neutralizing and non-neutralizing activities. The protection in MVA-ID group correlated positively with serum neutralizing and antibody-dependent phagocytic activities, and envelope-specific vaginal IgA; while the limited protection in MVA-IM group correlated only with serum neutralizing activity. MVA-ID immunizations induced greater germinal center Tfh and B cell responses, reduced the ratio of Th1 to Tfh cells in blood and showed lower activation of intermediate monocytes and inflammasome compared to MVA-IM immunizations. This lower innate activation correlated negatively with induction of Tfh responses. These data demonstrate that the MVA-ID vaccinations protect against intravaginal SHIV challenges by modulating the innate and T helper responses.
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| Overall design |
We vaccinated two groups of rhesus macaques with 10 animals per group using the DNA/MVA/Protein vaccine regimen. Animals in both groups were primed with DNA/SHIV-BG505-CD40L via the ID route and electroporation at weeks 0 and 8. At weeks 16 and 24, all animals were boosted with MVA/SHIV-BG505 delivered through either ID or IM routes.
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| Web link |
https://doi.org/10.1038/s41467-023-40430-7
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| Contributor(s) |
Bollimpelli VS, Reddy PB, Gangadhara S, Charles TP, Burton SL, Tharp GK, Styles TM, Labranche CC, Smith JC, Upadhyay AA, Sahoo A, Legere T, Shiferaw A, Velu V, Yu T, Tomai M, Vasilakos J, Kasturi SP, Shaw GM, Montefiori D, Bosinger SE, Kozlowski PA, Pulendran B, Derdeyn CA, Hunter E, Amara RR |
| Citation(s) |
37553348 |
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| Submission date |
Nov 30, 2022 |
| Last update date |
Oct 26, 2023 |
| Contact name |
Gregory K Tharp |
| E-mail(s) |
gktharp@emory.edu
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| Phone |
404-727-7797
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| Organization name |
Yerkes National Primate Research Center
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| Department |
Developmental and Cognitive Neuroscience
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| Lab |
Genomics Core
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| Street address |
954 Gatewood Dr
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| City |
Atlanta |
| State/province |
GA |
| ZIP/Postal code |
30329-4208 |
| Country |
USA |
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| Platforms (1) |
| GPL23804 |
Illumina HiSeq 3000 (Macaca mulatta) |
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| Samples (97)
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| Relations |
| BioProject |
PRJNA907055 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE219118_19067_Satish_DESeq2_RawCountsAnalysis.txt.gz |
2.3 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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