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Series GSE21920 Query DataSets for GSE21920
Status Public on Apr 20, 2011
Title affy_syringae_malaga_athaliana-Role of different proteases in plant defence
Organism Arabidopsis thaliana
Experiment type Expression profiling by array
Summary affy_syringae_malaga_athaliana - affy_syringae_malaga_athaliana - - Pseudomonas syringae is a plant pathogenic bacterium that relies on a type III secretion system (T3SS) to cause disease in susceptible hosts and to trigger defence in resistant plants. The T3SS translocates effector proteins directly inside the plant cell. Some P. syringae pathovars translocate the effector HopZ1a, which is recognized in Arabidopsis, triggering the hypersensitive response (HR). This resistance does not depend on the usual defence pathways involved in resistance against other avirulence effectors, so it would be very helpful to know the transcriptomic events that take place in the context of a hypersensitive response triggered by HopZ1a. ULP genes codifies for plant SUMO-proteases. Recent studies by our group revealed that they could be involved in plant defense against microorganisms.-- We infiltrated Arabidopsis thaliana ecotype Columbia wild type with 10mM MgCl2 (as mock inoculation), the virulent pathogen Pseudomonas syringae pv. tomato (Pto), Pto expressing the effector HopZ1a and Pto expressing a HopZ1a derivative carrying a point mutation in a residue essential for the cystein-protease activity. We also grew ulp1c/ulp1d mutant plants to analyze them either without treatment or inoculated with MgCl2 (as mock) and Pto wt.

Keywords: gene knock out,normal vs antisens mutant comparison
 
Overall design 24 arrays - ATH1
Web link http://urgv.evry.inra.fr/CATdb
 
Contributor(s) Huguet S, Macho A, Balzergue S, Martin-Magniette ML
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Submission date May 20, 2010
Last update date Aug 15, 2018
Contact name stephanie huguet
E-mail(s) huguet@evry.inra.fr
Organization name inra
Street address 2 rue gaston cremieux
City evry
ZIP/Postal code 91000
Country France
 
Platforms (1)
GPL198 [ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array
Samples (24)
GSM545363 colmut_3 with array type ATH1 from Affymetrix
GSM545364 colhop_2 with array type ATH1 from Affymetrix
GSM545365 ulp_1 with array type ATH1 from Affymetrix
Relations
Affiliated with GSE69995
BioProject PRJNA126801

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE21920_RAW.tar 43.0 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
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