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| Status |
Public on Dec 06, 2022 |
| Title |
Magnesium modulates Bacillus subtilis cell division frequency |
| Organism |
Bacillus subtilis subsp. subtilis str. 168 |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
By chance, we discovered a window of extracellular magnesium (Mg2+) availability that modulates Bacillus subtilis division frequency without affecting growth rate. In this window, cells grown with excess Mg2+ produce shorter cells than those grown in unsupplemented medium. The Mg2+-responsive adjustment in cell length occurs in both rich and minimal media and in domesticated and undomesticated strains. Of other divalent cations tested, manganese (Mn2+) and zinc (Zn2+) also resulted in cell shortening, but only at concentrations that affected growth. Cell length decreased proportionally with increasing Mg2+ from 0.2 mM to 4.0 mM, with little or no detectable change in labile, intracellular Mg2+ based on a riboswitch reporter. Cells grown in excess Mg2+ had fewer nucleoids and possessed more FtsZ-rings per unit cell length, consistent with increased division frequency. Remarkably, when shifting cells from unsupplemented to supplemented medium, more than half of the cell length decrease occurred in the first 10 min, consistent with rapid division onset. Relative to unsupplemented cells, cells growing at steady-state with excess Mg2+ showed enhanced expression of a large number of SigB-regulated genes and activation of the Fur, MntR, and Zur regulons. Thus, by manipulating the availability of one nutrient, we were able to uncouple growth rate from division frequency and identify transcriptional changes suggesting cell division is accompanied by the general stress response and an enhanced demand to sequester and/or increase uptake of iron, Mn2+, and Zn2+.
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| Overall design |
We performed gene expression profiling analysis using data obtained from RNA-seq of three biological repeats with or without Mg supplement at exponential stage.
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| Contributor(s) |
Guo T, Herman J |
| Citation(s) |
36515540 |
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| Submission date |
Dec 02, 2022 |
| Last update date |
Dec 16, 2022 |
| Contact name |
Jennifer Herman |
| E-mail(s) |
jkherman@tamu.edu
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| Organization name |
Texas A&M University
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| Department |
Biochemistry & Biophysics
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| Lab |
BCBP 305
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| Street address |
300 Olsen blvd
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| City |
College Station |
| State/province |
Texas |
| ZIP/Postal code |
77840 |
| Country |
USA |
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| Platforms (1) |
| GPL24119 |
Illumina HiSeq 4000 (Bacillus subtilis subsp. subtilis str. 168) |
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| Samples (6)
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| GSM6778090 |
Bs168 without Mg supplement, biol rep 1 |
| GSM6778091 |
Bs168 without Mg supplement, biol rep 2 |
| GSM6778092 |
Bs168 without Mg supplement, biol rep 3 |
| GSM6778093 |
Bs168 with 10mM MgCl2 supplement, biol rep 1 |
| GSM6778094 |
Bs168 with 10mM MgCl2 supplement, biol rep 2 |
| GSM6778095 |
Bs168 with 10mM MgCl2 supplement, biol rep 3 |
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| Relations |
| BioProject |
PRJNA907752 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE219221_RAW.tar |
220.0 Kb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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