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| Status |
Public on Mar 27, 2024 |
| Title |
scRNA-seq of ferret cortical germinal zones from splenial gyrus and lateral sulcus (Borrell lab) |
| Organism |
Mustela putorius |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Diversity of cortical radial glia cells (RGCs) and their complex relationships to generate neurons in species with expanded germinal zones and a folded cortex, remains unclear. We used single-cell RNA sequencing (scRNA-seq) of microdissected cortical germinal layers (ventricular zone (VZ) and outer subventricular zone (OSVZ)), from two cortical regions (splenial gyrus (SG) and neighboring lateral sulcus (LS)) at two critical time points for ferret cortex development (embryonic day (E) 34 and postnatal day (P) 1) to distinguish the molecular diversity of progenitors and newborn neurons, and study their transcriptomic trajectories.
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| Overall design |
Individually microdissected germinal zones from living brain slices were isolated from the six following conditions (E34_VZ_SG, E34_VZ_LS, P1_VZ_SG, P1_VZ_LS, P1_OSVZ_SG, P1_VZ_LS) and processed for scRNA-seq. We obtained each condition from 3 independent biological replicas, each replica profiled individually and containing cells from 2-4 sibling E34 embryos or 1 P1 kit, from a total of 9 litters.
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| Web link |
https://doi.org/10.1126/sciadv.adn9998
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| Contributor(s) |
Del-Valle-Anton L, Amin S, Cimino D, Neuhaus F, Dvoretskova E, Fernández V, Babal YK, Garcia-Frigola C, Prieto-Colomina A, Murcia-Ramón R, Nomura Y, Cárdenas A, Feng C, Moreno-Bravo J, Götz M, Mayer C, Borrell V |
| Citation(s) |
38536915, 38838158 |
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| Submission date |
Dec 02, 2022 |
| Last update date |
Jun 26, 2024 |
| Contact name |
Lucia Del-Valle-Anton |
| Organization name |
Instituto de Neurociencias, Consejo Superior de Investigaciones Científicas & Universidad Miguel Hernández
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| Lab |
Borrell Lab
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| Street address |
Av. Santiago Ramón y Cajal s/n
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| City |
Sant Joan d’Alacant |
| ZIP/Postal code |
03550 |
| Country |
Spain |
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| Platforms (1) |
| GPL32908 |
Illumina HiSeq 2500 (Mustela putorius) |
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| Samples (18)
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| GSM6779220 |
E34_VZ_LS, rep1 |
| GSM6779221 |
E34_VZ_LS, rep2 |
| GSM6779222 |
E34_VZ_LS, rep3 |
| GSM6779223 |
P1_VZ_SG, rep1 |
| GSM6779224 |
P1_VZ_SG, rep2 |
| GSM6779225 |
P1_VZ_SG, rep3 |
| GSM6779226 |
P1_VZ_LS, rep1 |
| GSM6779227 |
P1_VZ_LS, rep2 |
| GSM6779228 |
P1_VZ_LS, rep3 |
| GSM6779229 |
P1_OSVZ_SG, rep1 |
| GSM6779230 |
P1_OSVZ_SG, rep2 |
| GSM6779231 |
P1_OSVZ_SG, rep3 |
| GSM6779232 |
P1_OSVZ_LS, rep1 |
| GSM6779233 |
P1_OSVZ_LS, rep2 |
| GSM6779234 |
P1_OSVZ_LS, rep3 |
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| This SubSeries is part of SuperSeries: |
| GSE234305 |
Multiple parallel cell lineages in the developing mammalian cerebral cortex |
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| Relations |
| BioProject |
PRJNA907812 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE219254_RAW.tar |
570.1 Mb |
(http)(custom) |
TAR (of MTX, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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