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| Status |
Public on Feb 06, 2011 |
| Title |
Mapping replication-initiation proteins ORC1 and CDC6 in Arabidopsis thaliana |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
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| Summary |
Genomic integrity requires faithful chromosome duplication. Origins of replication are the genomic sites where DNA replication initiates in every cell cycle. There are multiple origins scattered throughout the eukaryotic genome whose genome-wide identification has been a hard challenge, especially in multicellular organisms. Thus, very little is known on the distinctive features of origins in terms of DNA sequence and chromatin context at a genomic scale. As part of a project for profiling replication origins in Arabidopsis thaliana, we have performed ChIP-chip analysis of the binding of ORC1 and CDC6, two proteins involved in initiation of DNA replication. Here, we provide the data of the ORC1-bound and CDC6-bound genomic sites using as control genomic DNA.
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| Overall design |
3 biological replicates of ORC1-binding sites-enriched DNA samples were compared against 3 biological replicates of fragmented genomic DNA (INPUT).
2 biological replicates of CDC6-binding-sites-enriched DNA samples were compared against 3 biological samples of fragmented genomic DNA (INPUT).
Results files linked below: Enrichment ratio calculated from normalized intensities and averaged over all replicates. Positive values indicate enrichment of the ChIP fraction versus the INPUT sample.
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| Contributor(s) |
Gutierrez C, Jacobsen SE |
| Citation(s) |
21297636 |
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| Submission date |
May 20, 2010 |
| Last update date |
May 09, 2017 |
| Contact name |
Juan Carlos Oliveros |
| Organization name |
CNB, CSIC
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| Street address |
Darwin 3
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| City |
Cantoblanco |
| State/province |
Madrid |
| ZIP/Postal code |
28049 |
| Country |
Spain |
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| Platforms (1) |
| GPL10977 |
[At35b_MR] Affymetrix Arabidopsis Tiling 1.0R Array |
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| Samples (2) |
| GSM545554 |
ORC1 samples vs INPUT (genomic DNA) |
| GSM545555 |
CDC6 samples vs INPUT (genomic DNA) |
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| Relations |
| BioProject |
PRJNA126817 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE21928_CDC6_vs_INPUT-FinalResults.txt.gz |
51.4 Mb |
(ftp)(http) |
TXT |
| GSE21928_ORC1_vs_INPUT-FinalResults.txt.gz |
51.2 Mb |
(ftp)(http) |
TXT |
| GSE21928_RAW.tar |
271.9 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data are available on Series record |
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