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| Status |
Public on Dec 31, 2010 |
| Title |
Characterization of pathogen specific expression of host immune response genes in Mycobacterium and Anaplasma spp. infection in ruminants |
| Platform organism |
Bos taurus |
| Sample organism |
Cervus elaphus |
| Experiment type |
Expression profiling by array
|
| Summary |
Anaplasma and Mycobacterium species are known to modify gene expression in ruminants. The objectives of this study were (a) to characterize global gene expression profiles in European red deer (Cervus elaphus) in response to Anaplasma ovis and A. ovis/Mycobacterium bovis/M. avium sub. paratuberculosis (MAP) infections, (b) to compare the expression of immune response genes between A. ovis- and A. ovis/M. bovis/MAP-infected deer, and (c) to characterize the differential expression of immune response genes identified in red deer in cattle infected with M. bovis and A. marginale. The results of this study showed that global gene differential expression in A. ovis- and A. ovis/M. bovis/MAP-infected deer results in the modification of common and pathogen-specific cellular biological processes. The differential expression of host immune response genes also showed pathogen-specific signatures and the effect of infection with multiple pathogens on red deer host immune response. These results suggested that intracellular bacteria from Anaplasma and Mycobacterium genera use similar mechanisms to infect and multiply within ruminant host cells while pathogen-specific mechanisms underline differences that could contribute to disease characterization and diagnosis in ruminants.
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| Overall design |
A gene expression pre analysis was made in deers naturally infected with Anaplasma ovis and Mycobacterium complex using Affymetrix Bos taurus microarray to detect differentialy expressed genes. The immune response genes with variation in expression were analyzed by real time RT-PCR in the same samples and a bigger group of deers. A real time RT-PCR analysis was also made in Bos taurus naturally infected with Anaplasma marignale.
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| Contributor(s) |
Galindo RC, Ayllon N, Carta T, Gortázar C, de la Fuente J |
| Citation(s) |
20952064 |
| Submission date |
May 23, 2010 |
| Last update date |
Dec 17, 2012 |
| Contact name |
Ruth Cecilia Galindo |
| Organization name |
Instituto de Investigacion en Recursos Cinegéticos
|
| Department |
Animal Health
|
| Lab |
Genomic
|
| Street address |
Ronda de Toledo s/n
|
| City |
Ciudad Real |
| State/province |
Ciudad Real |
| ZIP/Postal code |
13005 |
| Country |
Spain |
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| Platforms (1) |
| GPL2112 |
[Bovine] Affymetrix Bovine Genome Array |
|
| Samples (7)
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| GSM546237 |
Cervus elaphus naturally infected with Anaplasma ovis sample 1 |
| GSM546238 |
Cervus elaphus naturally infected with Anaplasma ovis sample 2 |
| GSM546239 |
Cervus elaphus naturally infected with Anaplasma ovis sample 3 |
| GSM546240 |
Cervus elaphus naturally infected with Mycobacterium complex sample 1 |
| GSM546241 |
Cervus elaphus naturally infected with Mycobacterium complex sample 2 |
| GSM546242 |
Cervus elaphus uninfected sample 1 |
| GSM546243 |
Cervus elaphus uninfected sample 2 |
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| Relations |
| BioProject |
PRJNA126781 |
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