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| Status |
Public on Aug 03, 2010 |
| Title |
Analysis of HeLa cells after transfection with miR-1 or miR-155, by microarray profiling |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
MicroRNAs (miRNAs) are endogenous ~22-nucleotide RNAs that mediate important gene-regulatory events by pairing to the mRNAs of protein-coding genes to direct their repression. Repression of these regulatory targets leads to decreased translational efficiency and/or decreased mRNA levels, but the relative contributions of these two outcomes have been largely unknown, particularly for endogenous targets expressed at low-to-moderate levels. Here, we use ribosome profiling to measure the overall effects on protein production and compare these to simultaneously measured effects on mRNA levels. For both ectopic and endogenous miRNA regulatory interactions, lowered mRNA levels account for most (≥84%) of the decreased protein production. These results show that changes in mRNA levels closely reflect the impact of miRNAs on gene expression and indicate that destabilization of target mRNAs is the predominant reason for reduced protein output.
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| Overall design |
Examine mRNA expression levels in HeLa cells transfected with miR-1 or miR-155, versus mock-transfected cells, at two different time points post-transfection.
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| Contributor(s) |
Guo H, Ingolia NT, Weissman JS, Bartel DP |
| Citation(s) |
20703300 |
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| Submission date |
May 26, 2010 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Huili Guo |
| E-mail(s) |
hguo@nus.edu.sg
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| Organization name |
National University of Singapore
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| Street address |
28 Medical Drive
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| City |
Singapore |
| ZIP/Postal code |
117456 |
| Country |
Singapore |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (6)
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| GSM546991 |
HeLa cells, mock-transfected, 32hr |
| GSM546992 |
HeLa cells, transfected with miR-155 duplex, 32hr |
| GSM546993 |
HeLa cells, transfected with miR-1 duplex, 32hr |
| GSM546994 |
HeLa cells, mock-transfected, 12hr |
| GSM546995 |
HeLa cells, transfected with miR-155 duplex, 12hr |
| GSM546996 |
HeLa cells, transfected with miR-1 duplex, 12hr |
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| This SubSeries is part of SuperSeries: |
| GSE22004 |
Mammalian microRNAs predominantly act to decrease target mRNA levels |
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| Relations |
| BioProject |
PRJNA129165 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE22002_RAW.tar |
27.5 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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