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| Status |
Public on Jul 15, 2010 |
| Title |
Feedback amplification of fibrosis through matrix stiffening and COX-2 suppression |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
We tested the hypothesis that increasing matrix stiffness on which normal human lung fibroblasts are grown promotes the expression of a fibrogenic cellular transcriptomic program.
Keywords: Human lung fibroblast, matrix stiffness, PTGS2, COX-2, Prostaglandin E2
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| |
|
| Overall design |
Total RNA extracted from normal human lung fibroblasts from 3 human subjects and separately grown on 5 discrete matrix stiffness conditions: 100, 400, 1600, 6400, 25600 Pascals.
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| |
|
| Contributor(s) |
Kho AT, Liu F, Mih JD, Tschumperlin DJ |
| Citation(s) |
20733059, 25502501 |
| Submission date |
May 26, 2010 |
| Last update date |
Jul 26, 2018 |
| Contact name |
Alvin T. Kho |
| E-mail(s) |
alvin_kho@hms.harvard.edu
|
| Phone |
617-919-2182
|
| Organization name |
Boston Children's Hospital
|
| Department |
Informatics Program
|
| Street address |
320 Longwood Avenue
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
|
| Samples (15)
|
| GSM547181 |
Donor 1 human lung fibroblast on 100 Pa stiffness gel |
| GSM547182 |
Donor 1 human lung fibroblast on 400 Pa stiffness gel |
| GSM547183 |
Donor 1 human lung fibroblast on 1600 Pa stiffness gel |
| GSM547184 |
Donor 1 human lung fibroblast on 6400 Pa stiffness gel |
| GSM547185 |
Donor 1 human lung fibroblast on 25600 Pa stiffness gel |
| GSM547186 |
Donor 2 human lung fibroblast on 100 Pa stiffness gel |
| GSM547187 |
Donor 2 human lung fibroblast on 400 Pa stiffness gel |
| GSM547188 |
Donor 2 human lung fibroblast on 1600 Pa stiffness gel |
| GSM547189 |
Donor 2 human lung fibroblast on 6400 Pa stiffness gel |
| GSM547190 |
Donor 2 human lung fibroblast on 25600 Pa stiffness gel |
| GSM547191 |
Donor 3 human lung fibroblast on 100 Pa stiffness gel |
| GSM547192 |
Donor 3 human lung fibroblast on 400 Pa stiffness gel |
| GSM547193 |
Donor 3 human lung fibroblast on 1600 Pa stiffness gel |
| GSM547194 |
Donor 3 human lung fibroblast on 6400 Pa stiffness gel |
| GSM547195 |
Donor 3 human lung fibroblast on 25600 Pa stiffness gel |
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| Relations |
| BioProject |
PRJNA127477 |
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