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| Status |
Public on Mar 02, 2023 |
| Title |
Changes in carbohydrate metabolism in alveolar epithelial type II cells in Ub-Cre and Hif1a loxp/loxp Ub cre mice in respnse to injurious mechanical ventilation (IMV) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by RT-PCR
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| Summary |
IMV induces transcription of key glycolytic enzymes in alveolar epithelial type II mice
IMV induced transcription of key glycolytic enzymes is HIF1A dependent
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| Overall design |
Mice were subjected to IMV (peak inspiratory pressure 45, PEEP 3, RR 80, FiO2 100%) or control ventilation (peak inspiratory pressure 12, PEEP 3, RR 80, FiO2 100%) , duration of mechanical ventilation 4 hours. After that alveolar type II epithelial cells were isolated form lungs
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| Contributor(s) |
Vohwinkel C |
| Citation(s) |
36326834 |
| Submission date |
Dec 05, 2022 |
| Last update date |
Mar 02, 2023 |
| Contact name |
Christine Vohwinkel |
| E-mail(s) |
christine.vohwinkel@cuanschutz.edu
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| Organization name |
University of Colorado
|
| Street address |
12700 E 19th Ave
|
| City |
Aurora |
| State/province |
COLORADO |
| ZIP/Postal code |
80205 |
| Country |
USA |
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| Platforms (1) |
| GPL32912 |
RT² Profiler™ PCR Array Mouse Glucose Metabolism |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA908840 |
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