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| Status |
Public on Jan 12, 2023 |
| Title |
Multimodal single nuclei profiling of human macular and peripheral retinal pigment epithelial cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
We sought to understand how the RPE confers region-specific disease susceptibility in the retina by examining heterogeneity within human donor RPE. We profiled RPE nuclei from the macular and peripheral retina using joint single-cell RNA and ATAC sequencing.
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| Overall design |
RPE nuclei were isolated from macular and peripheral regions of the posterior pole from four healthy human donors. Joint profiling of nuclear chromatin and transcript abundance was performed.
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| Contributor(s) |
Mullin NK, Mullins RF |
| Citation(s) |
36775060 |
| Submission date |
Dec 05, 2022 |
| Last update date |
Apr 13, 2023 |
| Contact name |
Nathaniel Kevin Mullin |
| Organization name |
University of Iowa
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| Street address |
375 Newton Road
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| City |
Iowa City |
| State/province |
IA |
| ZIP/Postal code |
52246 |
| Country |
USA |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (16)
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| GSM6792822 |
donor 2, RPE, peripheral, gex |
| GSM6792823 |
donor 3, RPE, macula, gex |
| GSM6792824 |
donor 3, RPE, peripheral, gex |
| GSM6792825 |
donor 4, RPE, macula, gex |
| GSM6792826 |
donor 4, RPE, peripheral, gex |
| GSM6792827 |
donor 1, RPE, macula, atac |
| GSM6792828 |
donor 1, RPE, peripheral, atac |
| GSM6792829 |
donor 2, RPE, macula, atac |
| GSM6792830 |
donor 2, RPE, peripheral, atac |
| GSM6792831 |
donor 3, RPE, macula, atac |
| GSM6792832 |
donor 3, RPE, peripheral, atac |
| GSM6792833 |
donor 4, RPE, macula, atac |
| GSM6792834 |
donor 4, RPE, peripheral, atac |
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| Relations |
| BioProject |
PRJNA908876 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE220155_RAW.tar |
40.1 Mb |
(http)(custom) |
TAR (of CSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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