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Series GSE22032 Query DataSets for GSE22032
Status Public on Oct 30, 2010
Title Defining Cell Fate And Embryonic Genome Activation By Global Single-Cell cDNA Analysis of Blastomeres From 5-to 8-Cell Human Embryos.
Organism Homo sapiens
Experiment type Expression profiling by array
Summary To determine blastomere fate and embryonic genome activation (EGA) at 5- to 8-cell stage human embryos by global gene expression profile of amplified cDNA from blastomeres at the single cell level
 
Overall design Forty-nine blastomeres from 5-, 6- and 8-cell human embryos were analyzed through whole genome wide analysis following an efficient cDNA amplification protocol (Kurimoto et al., 2007) with slight modifications. Single biopsied blastomeres were also compared with two amplified inner cell masses and two trophectoderms from blastocysts.
 
Contributor(s) Galán A, Montaner D, Póo ME, Dopazo J, Simón C
Citation(s) 21049019, 23614026
Submission date May 27, 2010
Last update date Feb 22, 2018
Contact name Amparo Galan
E-mail(s) agalan@cipf.es
Organization name Prince Felipe Research Centre (CIPF)
Street address Avda Autopista del Saler 16, 3
City Valencia
ZIP/Postal code 46021
Country Spain
 
Platforms (1)
GPL4133 Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version)
Samples (53)
GSM547695 blastomere from 5-cell embryo E1.M1
GSM547696 blastomere from 5-cell embryo E2.M1
GSM547697 blastomere from 5-cell embryo E3.M1
Relations
BioProject PRJNA127463

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE22032_RAW.tar 476.9 Mb (http)(custom) TAR (of TXT)
GSE22032_data_normalized_allgenes.txt.gz 10.6 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record
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