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| Status |
Public on May 29, 2010 |
| Title |
Small RNAs in transgenic TaNAM-RNAi wheat plants |
| Organism |
Triticum aestivum |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
To explore the effect of stable RNAi on the small RNA (sRNA) population in wheat, we constructed a sRNA library from hexaploid wheat that expresses an RNAi construct under the 35S promoter that targets the endogenous NO APICAL MERISTEM (TaNAM) gene. The presence of this RNAi transgene causes a 40% reduction in expression of the target genes as measured by quantitative RT-PCR and significantly delays senescence and reduces remobilization of N, Fe, and Zn to the grain.
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| Overall design |
RNA was extracted from flag-leaves of transgenic NAM-RNAi plants at 12 days post-anthesis. RNA was collected from a pool of flag-leaves from four plants.
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| Contributor(s) |
Cantu D, Michelmore R, Dubcovsky J |
| Citation(s) |
20584339 |
| |
| Submission date |
May 28, 2010 |
| Last update date |
May 15, 2019 |
| Contact name |
Dario Cantu |
| E-mail(s) |
dacantu@ucdavis.edu
|
| Organization name |
UC Davis
|
| Department |
Plant Sciences
|
| Street address |
One Shields Ave
|
| City |
Davis |
| State/province |
CA |
| ZIP/Postal code |
95616 |
| Country |
USA |
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| Platforms (1) |
| GPL10467 |
Illumina Genome Analyzer (Triticum aestivum) |
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| Samples (1) |
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| Relations |
| SRA |
SRP002588 |
| BioProject |
PRJNA128989 |
