 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jul 29, 2023 |
| Title |
Transcriptional analysis in multiple barley varieties identifies signatures of waterlogging response |
| Organism |
Hordeum vulgare |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Waterlogging leads to major crop losses globally, particularly for waterlogging sensitive crops such as barley. Waterlogging reduces oxygen availability and results in additional stresses, leading to the activation of hypoxia and stress response pathways that promote plant survival. Although certain barley varieties have been shown to be more tolerant to waterlogging than others and some tolerance-related QTLs have been identified, the molecular mechanisms underlying this trait are mostly unknown. Transcriptomics approaches can provide very valuable information for our understanding of waterlogging tolerance. Here, we surveyed 21 barley varieties for the differential transcriptional activation of conserved hypoxia-response genes under waterlogging, and selected five varieties with different levels of induction of core hypoxia-response genes. We further characterized their phenotypic response to waterlogging in terms of shoot and root traits. RNA-sequencing to evaluate the genome-wide transcriptional responses to waterlogging of these selected varieties led to the identification of a set of 98 waterlogging-response genes common to the different datasets. Many of these genes are orthologs of the so-called ‘core hypoxia response genes’, thus highlighting the conservation of plant responses to waterlogging. Hierarchical clustering analysis also identified groups of genes with intrinsic differential expression between varieties prior to waterlogging stress. These genes could constitute interesting candidates to study ‘predisposition’ to waterlogging tolerance or sensitivity in barley.
|
| |
|
| Overall design |
Differential gene expression analysis from RNA-seq from 10-day old roots from 5 barley varieties (Golden Promise, Infinity, Passport, Pilastro, Regina) under waterlogged and control conditions, with 3 replicates per sample.
|
| Web link |
https://onlinelibrary.wiley.com/doi/10.1002/pld3.518
|
| |
|
| Contributor(s) |
Miricescu A, Brazel A, Beegan J, Wellmer F, Graciet E |
| Citation(s) |
37577136 |
| |
| Submission date |
Dec 08, 2022 |
| Last update date |
Nov 06, 2023 |
| Contact name |
Ailbhe Jane Brazel |
| E-mail(s) |
Ailbhe.brazel@mu.ie
|
| Organization name |
Maynooth University
|
| Department |
Biology Department
|
| Lab |
Graciet Lab
|
| Street address |
Maynooth
|
| City |
Co. Kildare |
| ZIP/Postal code |
W23 F2H6 |
| Country |
Ireland |
| |
|
| Platforms (1) |
|
| Samples (30)
|
| GSM6806510 |
Golden Promise, Waterlogged, Replicate 1 |
| GSM6806511 |
Golden Promise, Waterlogged, Replicate 2 |
| GSM6806512 |
Golden Promise, Waterlogged, Replicate 3 |
| GSM6806513 |
Infinity, Control, Replicate 1 |
| GSM6806514 |
Infinity, Control, Replicate 2 |
| GSM6806515 |
Infinity, Control, Replicate 3 |
| GSM6806516 |
Infinity, Waterlogged, Replicate 1 |
| GSM6806517 |
Infinity, Waterlogged, Replicate 2 |
| GSM6806518 |
Infinity, Waterlogged, Replicate 3 |
| GSM6806519 |
Passport, Control, Replicate 1 |
| GSM6806520 |
Passport, Control, Replicate 2 |
| GSM6806521 |
Passport, Control, Replicate 3 |
| GSM6806522 |
Passport, Waterlogged, Replicate 1 |
| GSM6806523 |
Passport, Waterlogged, Replicate 2 |
| GSM6806524 |
Passport, Waterlogged, Replicate 3 |
| GSM6806525 |
Pilastro, Control, Replicate 1 |
| GSM6806526 |
Pilastro, Control, Replicate 2 |
| GSM6806527 |
Pilastro, Control, Replicate 3 |
| GSM6806528 |
Pilastro, Waterlogged, Replicate 1 |
| GSM6806529 |
Pilastro, Waterlogged, Replicate 2 |
| GSM6806530 |
Pilastro, Waterlogged, Replicate 3 |
| GSM6806531 |
Regina, Control, Replicate 1 |
| GSM6806532 |
Regina, Control, Replicate 2 |
| GSM6806533 |
Regina, Control, Replicate 3 |
| GSM6806534 |
Regina, Waterlogged, Replicate 1 |
| GSM6806535 |
Regina, Waterlogged, Replicate 2 |
| GSM6806536 |
Regina, Waterlogged, Replicate 3 |
|
| Relations |
| BioProject |
PRJNA910139 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE220532_All_Morexv3_gene_count_matrix.csv.gz |
1.1 Mb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
|
|
|
|
 |
Less...
More...