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| Status |
Public on Aug 01, 2023 |
| Title |
Measuring the effect of deoxycholate on Bacteroides fragilis gene expression |
| Organism |
Bacteroides fragilis |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We used RNAseq as an approach to assess the B. fragilis strain p207 response to the bile salt, deoxycholic acid (DC). A 6min DC exposure dramatically remodeled the transcriptome with approximately 20% of transcripts exhibiting significant changes. Chaperones and efflux systems were among the most highly induced genes, while protein synthesis genes were strongly repressed by DC.
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| Overall design |
RNA-seq data were collected on triplicate samples of B. fragilis RNA from: 1) cells not exposed to deoxycholate (DC), 2) cells exposed to 0.01% DC for 6 minutes, and 3) cells exposed to 0.01% DC for 20 minutes.
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| Contributor(s) |
Fiebig A, Eren M, Sogin M, Chang E, Crosson S |
| Citation(s) |
37214927, 38063424 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| RC2 DK122394 |
Host and microbial basis of human ulcerative colitis and pouchitis: Identification, role, mechanisms, and resource development of host susceptibility and pathobiont factors |
UNIVERSITY OF CHICAGO |
EUGENE B CHANG |
|
| Submission date |
Dec 12, 2022 |
| Last update date |
Jan 29, 2024 |
| Contact name |
Sean Crosson |
| E-mail(s) |
crosson4@msu.edu
|
| Phone |
5178845345
|
| Organization name |
Michigan State University
|
| Department |
Dept. Microbiology and Molecular Genetics
|
| Street address |
567 Wilson Rd
|
| City |
East Lansing |
| State/province |
Michigan |
| ZIP/Postal code |
48824 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL32937 |
NextSeq 2000 (Bacteroides fragilis) |
|
| Samples (9)
|
| GSM6809653 |
B. fragilis - deoxycholate 6 min - rep 1 |
| GSM6809654 |
B. fragilis - deoxycholate 6 min - rep 2 |
| GSM6809655 |
B. fragilis - deoxycholate 6 min - rep 3 |
| GSM6809656 |
B. fragilis - deoxycholate 20 min - rep 1 |
| GSM6809657 |
B. fragilis - deoxycholate 20 min - rep 2 |
| GSM6809658 |
B. fragilis - deoxycholate 20 min - rep 3 |
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| Relations |
| BioProject |
PRJNA911175 |
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