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| Status |
Public on Jan 01, 2023 |
| Title |
Oral administration of engineered yeast produced H5HA-based vaccines |
| Organism |
Gallus gallus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
H5N8 influenza virus is a highly pathogenic pathogen for the poultry and human. Vaccination is the most effective method to control the spread of the virus right now. The traditional inactivated vaccine, though well developed and used widely, is laborious during application and more interests are stimulated in developing alternative approaches. In this study, we developed three HA gene-based yeast vaccines and our experimental results demonstrated that all of these vaccines elicited the humoral immunity, inhibited viral load in the chicken tissues, and provided protective efficacy partially due to the high dose of H5N8 virus. Molecular mechanism studies suggested that, compared to the traditional inactivated vaccine, our engineered yeast vaccines reshaped the immune cell microenvironment in bursa of Fabriciu to promote the defense and immune responses. Analysis of gut microbiota further suggested that oral administration of engineered ST1814G/H5HA yeast vaccines increased the diversity of gut microbiota and the increasement of Reuteri and Muciniphila might benefit the recovery from influenza virus infection. These results provide strong evidences for further clinical use of these engineered yeast vaccines in poultry.
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| Overall design |
At the 6th day after the third vaccination, three immunized chickens were randomly selected in each group. The total RNA of bursa of Fabricius were isolated and sent to RNA-seq. The genes expression of chickens bursa of Fabricius with ST1814G treat, ST1814G/H5HA treat, and Inactivated vaccine treated were examined, respectively.
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| Contributor(s) |
Huang J, Li Z, Xie R |
| Citation(s) |
37143654 |
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| Submission date |
Dec 12, 2022 |
| Last update date |
Aug 02, 2023 |
| Contact name |
Zexing Li |
| E-mail(s) |
lizexing_21@tju.edu.cn
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| Organization name |
Tianjin University
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| Department |
School of Life Sciences
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| Street address |
No.92 Weijin road
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| City |
Tianjin |
| ZIP/Postal code |
300072 |
| Country |
China |
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| Platforms (1) |
| GPL19005 |
Illumina HiSeq 2500 (Gallus gallus) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA911230 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE220714_RAW.tar |
6.3 Mb |
(http)(custom) |
TAR (of TXT) |
| GSE220714_gene_count_matrix.csv.gz |
543.2 Kb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
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