|
| Status |
Public on Jul 20, 2023 |
| Title |
Establishment of bovine trophoblast stem cells [WGBS] |
| Organism |
Bos taurus |
| Experiment type |
Methylation profiling by high throughput sequencing
|
| Summary |
Here we report that a chemical cocktail (LCDM: hLIF, CHIR99021, DiM and MiH) previously reported for extended potential pluripotent stem cells enables the de novo derivation and long-term culture of bovine trophoblast stem cells (TSCs). Bovine TSCs exhibit transcriptomic and epigenetic features characteristic of trophectoderm cells from bovine embryos and retain developmental potency to differentiate into functional trophoblasts in vitro and in vivo
|
| |
|
| Overall design |
Whole genome bisulfite sequencing analysis was used to determined genome-scale DNA methylation patterns of bTSC, D7_TE, and D14_TE.
|
| |
|
| Contributor(s) |
Jiang Z, Wang Y, Zhu L |
| Citation(s) |
37146606 |
| Submission date |
Dec 14, 2022 |
| Last update date |
Jul 21, 2023 |
| Contact name |
Zongliang Jiang |
| E-mail(s) |
z.jiang1@ufl.edu
|
| Phone |
352-273-8080
|
| Organization name |
University of Florida
|
| Department |
Dept. of Animal Sciences
|
| Street address |
2033 Mowry Rd, Cancer & Genetics Research Bldg, Room 303
|
| City |
Gainesville |
| State/province |
Florida |
| ZIP/Postal code |
32610 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL19172 |
Illumina HiSeq 2500 (Bos taurus) |
|
| Samples (8)
|
|
| This SubSeries is part of SuperSeries: |
| GSE220925 |
Establishment of bovine trophoblast stem cells |
|
| Relations |
| BioProject |
PRJNA912082 |
Less...
More...