|
| Status |
Public on Jan 17, 2023 |
| Title |
The impact of iron depletion on the transcriptome of Enterococcus faecalis |
| Organism |
Enterococcus faecalis OG1RF |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To identify the genes and pathways utilized by E. faecalis to grow under iron starvation, we used RNA deep sequencing (RNA-seq) to compare the transscriptome of the parent strain OG1RF grown to mid-log in the chemically defined FMC medium with or without the addition of FeSO4 as an iron source.
|
| |
|
| Overall design |
E. faecalis OG!RF cells were grown to mid-log phase in chemically defined FMC medium with (Complete) or without (Minus Fe) supplementation with 80uM FeSO4 as a source of iron. Total RNA was isolated and prepared for RNA-Seq analysis.
|
| |
|
| Contributor(s) |
Kajfasz JK, Brunson DN, Colomer-Winter C, Lam LN, Lemos JA |
| Citation(s) |
36912636 |
| |
| Submission date |
Dec 15, 2022 |
| Last update date |
Apr 19, 2023 |
| Contact name |
Jessica K Kajfasz |
| E-mail(s) |
jkajfasz@dental.ufl.edu
|
| Organization name |
University of Florida
|
| Department |
Oral Biology
|
| Lab |
Lemos-Abranches
|
| Street address |
1395 Center Drive
|
| City |
Gainesville |
| State/province |
FL |
| ZIP/Postal code |
32610 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL32954 |
Illumina NextSeq 500 (Enterococcus faecalis OG1RF) |
|
| Samples (6)
|
| GSM6841247 |
E. faecalis OG1RF grown in FMC medium supplemented with 80uM FeSO4. Replicate 1 |
| GSM6841248 |
E. faecalis OG1RF grown in FMC medium supplemented with 80uM FeSO4. Replicate 2 |
| GSM6841249 |
E. faecalis OG1RF grown in FMC medium supplemented with 80uM FeSO4. Replicate 3 |
| GSM6841250 |
E. faecalis OG1RF grown in FMC medium lacking FeSO4. Replicate 1 |
| GSM6841251 |
E. faecalis OG1RF grown in FMC medium lacking FeSO4. Replicate 2 |
| GSM6841252 |
E. faecalis OG1RF grown in FMC medium lacking FeSO4. Replicate 3 |
|
| Relations |
| BioProject |
PRJNA912678 |
Less...
More...