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Series GSE22106 Query DataSets for GSE22106
Status Public on Jun 03, 2010
Title Hydrocortisone induces changes in gene expression and differentiation of immature human enterocytes
Organism Homo sapiens
Experiment type Expression profiling by array
Summary It It is known that functional maturation of the small intestine occurring during the weaning period is facilitated by glucocorticoids (such as hydrocortisone, HC) including the increased expression of digestive hydrolases. However, the molecular mechanism(s) are not well understood, particularly in human gut. Here we report a microarray analysis of HC- induced changes in gene expression in H4 (a human fetal small intestinal epithelial cell line well-characterized in numerous previous studies). This study identified a large number of HC-affected genes, some involved in metabolism, cell cycle regulation, cell polarity, tight junction formation, and interactions with extracellular matrices. These effects could play an important role in HC-mediated enterocyte maturation in vivo and in vitro.
 
Overall design hydrocortisone effect on gene expression prlfile in H4 cells over time (no HC, HC_12h, HC_24h, HC_48h, HC_5d and T84). Six samples are analyzed. Control sample is H4 without HC.
 
Contributor(s) Lu L, Walker WA
Citation(s) 21148402, 27056727
Submission date Jun 02, 2010
Last update date Oct 03, 2019
Contact name Lei Lu
E-mail(s) lul@helix.mgh.harvard.edu
Organization name Massachusetts General Hospital
Street address 114, 16th Street
City Charlestown
ZIP/Postal code 02129
Country USA
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (6)
GSM549595 H4_ctrl
GSM549596 H4_HC12h
GSM549597 H4_HC24h
Relations
BioProject PRJNA128911

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE22106_RAW.tar 28.7 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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