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| Status |
Public on Jun 14, 2023 |
| Title |
Exogenous interleukin-1 beta stimulation regulates equine tenocyte function and gene expression which can only be rescued by pharmacological inhibition of interleukin 1 receptor, but not nuclear factor kappa B, signalling |
| Organism |
Equus caballus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We investigated how Interleukin 1 beta impacts equine tenocyte function and global gene expression in 3D culture and determined if these effects could be rescued by pharmacologically inhibiting nuclear factor-κB or interleukin 1 signalling.
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| Overall design |
mRNA profiles of eight independent lines of adult tenocytes were generated by RNA sequencing, using Illumina NovaSeq6000. The sequence reads that passed quality filters were analyzed using the pseudoaligner Salmon and differential expression quantified using DESeq2. Gene ontology analysis was then conducted using Panther GoSlim and pathway analysis with Enrichr.
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| Web link |
https://link.springer.com/article/10.1007/s11010-023-04779-z
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| |
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| Contributor(s) |
Beaumont RB, Smith EJ, Zhou L, Marr N, Thorpe CT, Guest DJ |
| Citation(s) |
37314623, 38396222 |
| |
| Submission date |
Dec 19, 2022 |
| Last update date |
May 30, 2024 |
| Contact name |
Ross Eric Beaumont |
| E-mail(s) |
robeaumont@rvc.ac.uk
|
| Phone |
07312130009
|
| Organization name |
Royal Veterinary College
|
| Department |
Clinical science and services
|
| Lab |
Skeletal biology group
|
| Street address |
Hawkshead Ln
|
| City |
Hatfield |
| State/province |
Hertfordshire |
| ZIP/Postal code |
AL97TA |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL26749 |
Illumina NovaSeq 6000 (Equus caballus) |
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| Samples (19)
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| Relations |
| BioProject |
PRJNA914045 |
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