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| Status |
Public on Feb 15, 2023 |
| Title |
Inhibition of EZH2 causes retrotransposon derepression and immune activation in porcine lung alveolar macrophages [ChIP-seq] |
| Organism |
Sus scrofa |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Alveolar macrophages (AMs) form the first defense line against a variety of respiratory pathogens, and their immune response has profound impact on the outcome of respiratory infection. It is of critical importance to clarify the immune regulation in AMs. Enhancer of Zeste Homolog 2 (EZH2), which catalyzes the trimethylation of H3K27 for epigenetic repression, has gained increasing attentions about their roles in immune regulation. However, the molecular mechanism underlying the immune regulation role of EZH2 in AMs remains largely unclear. Using porcine 3D4/21 alveolar macrophage cells as model, this study combined transcriptomic and epigenomic techniques to decipher the molecular function of EZH2 in AMs. Transcriptomic comparison revealed that inhibition of EZH2 in AMs causes transcriptional activation of dozens of immune genes which indicates an enhanced antiviral state, and as expected, we found that the infection of influenza A viruses can be remarkably affected. Interestingly, about one hundred families of transposable elements, particularly LTRs/ERVs and LINEs which belong to retrotransposons, get derepressed after EZH2 inhibition. Given that derepression of ERVs promotes innate immune activation in many tumors through the “viral mimicry” mechanism, the derepression of retrotransposon in AMs may also contribute to the coincided immune activation after EZH2 inhibition. Overall, this study improves the mechanistical understanding on the EZH2-dependent immune regulation in AMs and provides novel insights for the epigenetic regulation of retrotransposons in pigs.
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| Overall design |
ChIP-Seq (H3K27ac) and RNA-Seq data are generated for porcine 3D4/21 alveolar macrophages with or without the treatment of the EZH2 inhibitor GSK126
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| Contributor(s) |
Zhang L, Sun M |
| Citation(s) |
36768720 |
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| Submission date |
Dec 21, 2022 |
| Last update date |
Feb 16, 2023 |
| Contact name |
Z LL |
| E-mail(s) |
MZ120201482@stu.yzu.edu.cn
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| Phone |
13283789136
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| Organization name |
yangzhouaxue
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| Street address |
shuangqiaojiedao
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| City |
yangzhou |
| ZIP/Postal code |
225009 |
| Country |
China |
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| Platforms (1) |
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| Samples (6)
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| GSM6873838 |
3D4/21, untreated, H3K27ac, rep1 [H3K27ac_00h_r1] |
| GSM6873839 |
3D4/21, untreated, H3K27ac, rep2 [H3K27ac_00h_r2] |
| GSM6873840 |
3D4/21, GSK126 treated for 48h, H3K27ac, rep1 [H3K27ac_48h_r1] |
| GSM6873841 |
3D4/21, GSK126 treated for 48h, H3K27ac, rep2 [H3K27ac_48h_r2] |
| GSM6873842 |
3D4/21, untreated, Input [Input_00h] |
| GSM6873843 |
3D4/21, GSK126 treated for 48h, Input [Input_48h] |
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| This SubSeries is part of SuperSeries: |
| GSE221479 |
Inhibition of EZH2 causes retrotransposon derepression and immune activation in porcine lung alveolar macrophages. |
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| Relations |
| BioProject |
PRJNA914633 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE221476_RAW.tar |
4.7 Mb |
(http)(custom) |
TAR (of NARROWPEAK) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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